Next Article in Journal
Study of the Distribution of Radiative Defects and Reabsorption of the UV in ZnO Nanorods-Organic Hybrid White Light Emitting Diodes (LEDs)
Previous Article in Journal / Special Issue
Influence of Surface Processing on the Biocompatibility of Titanium
Article Menu

Export Article

Open AccessArticle
Materials 2011, 4(7), 1249-1259; doi:10.3390/ma4071249

Migration Capacity and Viability of Human Primary Osteoblasts in Synthetic Three-dimensional Bone Scaffolds Made of Tricalciumphosphate

1
Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, University of Rostock, Rostock 18107, Germany
2
Department of Mechanical Engineering and Marine Technology, Chair of Fluid Technology and Microfluidics, University of Rostock, Rostock 18107, Germany
*
Author to whom correspondence should be addressed.
Received: 27 April 2011 / Revised: 1 June 2011 / Accepted: 30 June 2011 / Published: 8 July 2011
(This article belongs to the Special Issue Advances in Biomaterials 2011)
View Full-Text   |   Download PDF [404 KB, uploaded 8 July 2011]   |  

Abstract

In current therapeutic strategies, bone defects are filled up by bone auto- or allografts. Since they are limited by insufficient availability and donor site morbidity, it is necessary to find an appropriate alternative of synthetic porous bone materials. Because of their osteoconductive characteristics, ceramic materials like tricalciumphosphate (TCP) are suitable to fill up bone defects. Another advantage of TCP implants is the ability of patient-specific engineering. Objective of the present in-vitro study was to analyze the migration capacity and viability of human primary osteoblasts in porous three-dimensional TCP scaffolds in a static cell culture. To obtain data of the cellular supply with nutrients and oxygen, we determined the oxygen concentration and the pH value within the 3D scaffold compared to the surrounding medium using microsensors. After eight days of cultivation we found cells on all four planes. During incubation, the oxygen concentration within the scaffold decreased by approximately 8%. Furthermore, we could not demonstrate an increasing acidification in the core of the TCP scaffold. Our results suggest that osteoblasts could migrate and survive within the macroporous TCP scaffolds. The selected size of the macropores prevents overgrowth of cells, whereby the oxygen and nutrients supply is sufficiently guaranteed.
Keywords: human primary osteoblasts; tricalciumphosphat; scaffold; hypoxia; acidification; microsensors human primary osteoblasts; tricalciumphosphat; scaffold; hypoxia; acidification; microsensors
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jonitz, A.; Wieding, J.; Lochner, K.; Cornelsen, M.; Seitz, H.; Hansmann, D.; Bader, R. Migration Capacity and Viability of Human Primary Osteoblasts in Synthetic Three-dimensional Bone Scaffolds Made of Tricalciumphosphate. Materials 2011, 4, 1249-1259.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top