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Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study
Laboratory for Experimental Plastic Surgery, Institution of Clinical and Experimental Medicine, Faculty of Health Science, Linköpings Universitet, 581 83 Linköping, Sweden
Berzelius Clinical Research Center, Berzelius Science Park, 582 25 Linköping, Sweden
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Box 575, the Biomedical Centre, 751 23 Uppsala, Sweden
National Veterinary Institute, 751 89 Uppsala, Sweden
Department of Plastic-, Hand-, and Burn Surgery, University Hospital of Linköping, 581 85 Linköping, Sweden
* Author to whom correspondence should be addressed.
Received: 4 November 2009; in revised form: 17 November 2009 / Accepted: 19 November 2009 / Published: 20 November 2009
Abstract: Spider silk is an interesting biomaterial for medical applications. Recently, a method for production of recombinant spider silk protein (4RepCT) that forms macroscopic fibres in physiological solution was developed. Herein, 4RepCT and MersilkTM (control) fibres were implanted subcutaneously in rats for seven days, without any negative systemic or local reactions. The tissue response, characterised by infiltration of macrophages and multinucleated cells, was similar with both fibres, while only the 4RepCT-fibres supported ingrowth of fibroblasts and newly formed capillaries. This in vivo study indicates that 4RepCT-fibres are well tolerated and could be used for medical applications, e.g., tissue engineering.
Keywords: spider silk; recombinant; biocompatibility; in vivo
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Fredriksson, C.; Hedhammar, M.; Feinstein, R.; Nordling, K.; Kratz, G.; Johansson, J.; Huss, F.; Rising, A. Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study. Materials 2009, 2, 1908-1922.
Fredriksson C, Hedhammar M, Feinstein R, Nordling K, Kratz G, Johansson J, Huss F, Rising A. Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study. Materials. 2009; 2(4):1908-1922.
Fredriksson, Camilla; Hedhammar, My; Feinstein, Ricardo; Nordling, Kerstin; Kratz, Gunnar; Johansson, Jan; Huss, Fredrik; Rising, Anna. 2009. "Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study." Materials 2, no. 4: 1908-1922.