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Materials 2018, 11(2), 272; doi:10.3390/ma11020272

Nanostructure and Corresponding Quenching Efficiency of Fluorescent DNA Probes

State Key Laboratory of Separation Membranes and Membrane Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China
Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Author to whom correspondence should be addressed.
Received: 26 December 2017 / Revised: 25 January 2018 / Accepted: 5 February 2018 / Published: 9 February 2018
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Based on the fluorescence resonance energy transfer (FRET) mechanism, fluorescent DNA probes were prepared with a novel DNA hairpin template method, with SiO2 coated CdTe (CdTe/SiO2) core/shell nanoparticles used as the fluorescence energy donors and gold (Au) nanoparticles (AuNPs) as the energy acceptors. The nanostructure and energy donor/acceptor ratio in a probe were controlled with this method. The relationship between the nanostructure of the probes and FRET efficiency (quenching efficiency) were investigated. The results indicated that when the donor/acceptor ratios were 2:1, 1:1, and 1:2; the corresponding FRET efficiencies were about 33.6%, 57.5%, and 74.2%, respectively. The detection results indicated that the fluorescent recovery efficiency of the detecting system was linear when the concentration of the target DNA was about 0.0446–2.230 nmol/L. Moreover, the probes showed good sensitivity and stability in different buffer conditions with a low detection limit of about 0.106 nmol/L. View Full-Text
Keywords: fluorescent DNA probes; CdTe/SiO2 nanoparticles; Au nanoparticles; DNA hairpin template; nanostructure; quenching efficiency fluorescent DNA probes; CdTe/SiO2 nanoparticles; Au nanoparticles; DNA hairpin template; nanostructure; quenching efficiency

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MDPI and ACS Style

Guo, W.; Wei, Y.; Dai, Z.; Chen, G.; Chu, Y.; Zhao, Y. Nanostructure and Corresponding Quenching Efficiency of Fluorescent DNA Probes. Materials 2018, 11, 272.

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