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Materials 2018, 11(2), 272; https://doi.org/10.3390/ma11020272

Nanostructure and Corresponding Quenching Efficiency of Fluorescent DNA Probes

1
State Key Laboratory of Separation Membranes and Membrane Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China
2
Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
*
Author to whom correspondence should be addressed.
Received: 26 December 2017 / Revised: 25 January 2018 / Accepted: 5 February 2018 / Published: 9 February 2018
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Abstract

Based on the fluorescence resonance energy transfer (FRET) mechanism, fluorescent DNA probes were prepared with a novel DNA hairpin template method, with SiO2 coated CdTe (CdTe/SiO2) core/shell nanoparticles used as the fluorescence energy donors and gold (Au) nanoparticles (AuNPs) as the energy acceptors. The nanostructure and energy donor/acceptor ratio in a probe were controlled with this method. The relationship between the nanostructure of the probes and FRET efficiency (quenching efficiency) were investigated. The results indicated that when the donor/acceptor ratios were 2:1, 1:1, and 1:2; the corresponding FRET efficiencies were about 33.6%, 57.5%, and 74.2%, respectively. The detection results indicated that the fluorescent recovery efficiency of the detecting system was linear when the concentration of the target DNA was about 0.0446–2.230 nmol/L. Moreover, the probes showed good sensitivity and stability in different buffer conditions with a low detection limit of about 0.106 nmol/L. View Full-Text
Keywords: fluorescent DNA probes; CdTe/SiO2 nanoparticles; Au nanoparticles; DNA hairpin template; nanostructure; quenching efficiency fluorescent DNA probes; CdTe/SiO2 nanoparticles; Au nanoparticles; DNA hairpin template; nanostructure; quenching efficiency
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Guo, W.; Wei, Y.; Dai, Z.; Chen, G.; Chu, Y.; Zhao, Y. Nanostructure and Corresponding Quenching Efficiency of Fluorescent DNA Probes. Materials 2018, 11, 272.

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