Next Article in Journal
Anchoring ZnO Nanoparticles in Nitrogen-Doped Graphene Sheets as a High-Performance Anode Material for Lithium-Ion Batteries
Next Article in Special Issue
Stepwise Thermo-Responsive Amino Acid-Derived Triblock Vinyl Polymers: ATRP Synthesis of Polymers, Aggregation, and Gelation Properties via Flower-Like Micelle Formation
Previous Article in Journal
Al2O3 Coatings on Magnesium Alloy Deposited by the Fluidized Bed (FB) Technique
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Materials 2018, 11(1), 95; doi:10.3390/ma11010095

Post-Modified Polypeptides with UCST-Type Behavior for Control of Cell Attachment in Physiological Conditions

1
School of Pharmacy, the University of Nottingham, University Park, Nottingham NG7 2RD, UK
2
School of Materials, the University of Manchester, Manchester M13 9PL, UK
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 29 November 2017 / Revised: 31 December 2017 / Accepted: 5 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Temperature-Responsive Polymers)
View Full-Text   |   Download PDF [3599 KB, uploaded 17 January 2018]   |  

Abstract

Upper Critical Solution Temperature (UCST)-type thermally responsive polypeptides (TRPs) with phase transition temperatures around 37 °C in phosphate-buffered saline (PBS) buffer (pH 7.4, 100 mM) were prepared from poly(l-ornithine) hydrobromide and coated on non-tissue culture-treated plastic plates (nTCP). Cell adhesion was observed at temperatures above the phase transition temperature of the coating polymer (39 °C), while cell release was triggered when the culture temperature was switched to 37 °C. Approximately 65% of the attached cells were released from the surface within 6 h after changing the temperature, and more than 96% of the released cells were viable. Water contact angle measurements performed at 39 and 37 °C demonstrated that the surface hydrophobicity of the new TRP coatings changed in response to applied temperature. The cell attachment varied with the presence of serum in the media, suggesting that the TRP coatings mediated cell attachment and release as the underlying polymer surface changed conformation and consequently the display of adsorbed protein. These new TRP coatings provide an additional means to mediate cell attachment for application in cell-based tissue regeneration and therapies. View Full-Text
Keywords: UCST polymer; thermo-responsive polypeptides; controlled cell attachment UCST polymer; thermo-responsive polypeptides; controlled cell attachment
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Xue, X.; Thiagarajan, L.; Dixon, J.E.; Saunders, B.R.; Shakesheff, K.M.; Alexander, C. Post-Modified Polypeptides with UCST-Type Behavior for Control of Cell Attachment in Physiological Conditions. Materials 2018, 11, 95.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top