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Materials 2018, 11(1), 60; doi:10.3390/ma11010060

The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

1
Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany
2
Departament d’Enginyeria Quimica, Universitat Rovira i Virgili, 26 Avenue dels Paisos Catalans, 43007 Tarragona, Spain
3
Department of Chemistry and Biotechnology, School of Science, Swinburne University of Technology, P.O. Box 218, Hawthorn VIC 3122, Australia
4
School of Science, RMIT University, P.O. Box 2476, Melbourne VIC 3001, Australia
*
Author to whom correspondence should be addressed.
Received: 26 August 2017 / Revised: 1 December 2017 / Accepted: 27 December 2017 / Published: 31 December 2017
(This article belongs to the Special Issue Biocompatibility of Materials)
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Abstract

Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons. View Full-Text
Keywords: nerve growth factor (NGF); poly-L-lysine; fibronectin; laminin; PC12 neuronal differentiation nerve growth factor (NGF); poly-L-lysine; fibronectin; laminin; PC12 neuronal differentiation
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MDPI and ACS Style

Orlowska, A.; Perera, P.T.; Al Kobaisi, M.; Dias, A.; Nguyen, H.K.D.; Ghanaati, S.; Baulin, V.; Crawford, R.J.; Ivanova, E.P. The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells. Materials 2018, 11, 60.

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