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Materials 2017, 10(9), 1081; doi:10.3390/ma10091081

The Role of Controlled Surface Topography and Chemistry on Mouse Embryonic Stem Cell Attachment, Growth and Self-Renewal

1
School of Engineering, Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia
2
Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool L7 8TX, UK
3
Institute for Photonics and Advanced Sensing, University of Adelaide, Adelaide, SA 5000, Australia
*
Authors to whom correspondence should be addressed.
Received: 2 August 2017 / Revised: 12 September 2017 / Accepted: 12 September 2017 / Published: 14 September 2017
(This article belongs to the Special Issue Biocompatibility of Materials)
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Abstract

The success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation. View Full-Text
Keywords: plasma polymer; Polyallylamine; polyoctadiene; polyacrylic acid; nanotopography; Mouse embryonic stem cells; fibronectin; Extra Cellular Matrix physical cues; Extra Cellular Matrix chemical cues plasma polymer; Polyallylamine; polyoctadiene; polyacrylic acid; nanotopography; Mouse embryonic stem cells; fibronectin; Extra Cellular Matrix physical cues; Extra Cellular Matrix chemical cues
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Macgregor, M.; Williams, R.; Downes, J.; Bachhuka, A.; Vasilev, K. The Role of Controlled Surface Topography and Chemistry on Mouse Embryonic Stem Cell Attachment, Growth and Self-Renewal. Materials 2017, 10, 1081.

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