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Open AccessTechnical Note
Energies 2016, 9(3), 173; doi:10.3390/en9030173

Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

1
School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China
2
Hebei Province Environmental Monitoring Center, Shijiazhuang 050056, China
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Chen
Received: 21 December 2015 / Revised: 20 February 2016 / Accepted: 1 March 2016 / Published: 8 March 2016
(This article belongs to the Special Issue Algae Fuel 2015)
View Full-Text   |   Download PDF [1486 KB, uploaded 8 March 2016]   |  

Abstract

This study evaluated the effectiveness of pressure-assisted ozonation (PAO) in Chlorella vulgaris (C. vulgaris) cell disruption, and compared the disruption result with that of the ultrasonication (US) by using four quantification indicators: cell counting, ultra violet (UV) absorbance, turbidity and visible light absorbance. It was found that under the condition of 0.8 MPa and 80 cycles, PAO treatment achieved cell rupture of 80.3%, with the power of 1080 W and treatment time of 60 min, US achieved cell rupture of 83.8%. Cell counting was a reliable indicator and applicable to both PAO and US treatments. Turbidity and visible light absorbance gave similar results and featured as the simplest operation. UV absorbance reflected the metabolite release due to cell breakage; however, it was less reproducible when it was applied to quantify the cell rupture by PAO. Its trend indicated that during cell disruption metabolite degradation occurred, especially after significant rupture in the case of excessive PAO treatment. The cellular morphology of C. vulgaris cells during PAO and US treatments was investigated by scanning electron microscope (SEM) which certified that the cells damage was caused by both physical and chemical attack. View Full-Text
Keywords: cell disrupture; quantitative evaluation; Chlorella vulgaris (C. vulgaris); pressure-assisted ozonation (PAO); ultrasonication (US) cell disrupture; quantitative evaluation; Chlorella vulgaris (C. vulgaris); pressure-assisted ozonation (PAO); ultrasonication (US)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Huang, Y.; Qin, S.; Zhang, D.; Li, L.; Mu, Y. Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication. Energies 2016, 9, 173.

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