Next Article in Journal
Dynamic Power Management for Portable Hybrid Power-Supply Systems Utilizing Approximate Dynamic Programming
Next Article in Special Issue
Fast Pyrolysis of Four Lignins from Different Isolation Processes Using Py-GC/MS
Previous Article in Journal
Model-Based State Feedback Controller Design for a Turbocharged Diesel Engine with an EGR System
Previous Article in Special Issue
Techno-Economic Analysis of Bioethanol Production from Lignocellulosic Biomass in China: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover
Article Menu

Export Article

Open AccessArticle
Energies 2015, 8(6), 5040-5052; doi:10.3390/en8065040

Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

1
Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas 13083852, Brazil
2
Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources, Luleå University of Technology, Luleå SE 97187, Sweden
3
Department of Chemical Engineering & Material Science, Michigan State University, East Lansing, MI 48824, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas E. Amidon
Received: 23 March 2015 / Revised: 7 May 2015 / Accepted: 25 May 2015 / Published: 28 May 2015
(This article belongs to the Collection Bioenergy and Biofuel)
View Full-Text   |   Download PDF [392 KB, uploaded 28 May 2015]   |  

Abstract

Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species. View Full-Text
Keywords: single cell oil; lipid extraction; biodiesel; Lipomyces starkeyi; Rhodosporidium toruloides single cell oil; lipid extraction; biodiesel; Lipomyces starkeyi; Rhodosporidium toruloides
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bonturi, N.; Matsakas, L.; Nilsson, R.; Christakopoulos, P.; Miranda, E.A.; Berglund, K.A.; Rova, U. Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction. Energies 2015, 8, 5040-5052.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Energies EISSN 1996-1073 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top