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Energies 2014, 7(4), 2421-2435; doi:10.3390/en7042421
Article

Modeling of Clostridium tyrobutyricum for Butyric Acid Selectivity in Continuous Fermentation

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Received: 14 January 2014; in revised form: 27 March 2014 / Accepted: 4 April 2014 / Published: 17 April 2014
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Abstract: A mathematical model was developed to describe batch and continuous fermentation of glucose to organic acids with Clostridium tyrobutyricum. A modified Monod equation was used to describe cell growth, and a Luedeking-Piret equation was used to describe the production of butyric and acetic acids. Using the batch fermentation equations, models predicting butyric acid selectivity for continuous fermentation were also developed. The model showed that butyric acid production was a strong function of cell mass, while acetic acid production was a function of cell growth rate. Further, it was found that at high acetic acid concentrations, acetic acid was metabolized to butyric acid and that this conversion could be modeled. In batch fermentation, high butyric acid selectivity occurred at high initial cell or glucose concentrations. In continuous fermentation, decreased dilution rate improved selectivity; at a dilution rate of 0.028 h−1, the selectivity reached 95.8%. The model and experimental data showed that at total cell recycle, the butyric acid selectivity could reach 97.3%. This model could be used to optimize butyric acid production using C. tyrobutyricum in a continuous fermentation scheme. This is the first study that mathematically describes batch, steady state, and dynamic behavior of C. tyrobutyricum for butyric acid production.
Keywords: butyric acid; steady-state model; batch fermentation; continuous fermentation; Clostridium tyrobutyricum butyric acid; steady-state model; batch fermentation; continuous fermentation; Clostridium tyrobutyricum
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Du, J.; McGraw, A.; Hestekin, J.A. Modeling of Clostridium tyrobutyricum for Butyric Acid Selectivity in Continuous Fermentation. Energies 2014, 7, 2421-2435.

AMA Style

Du J, McGraw A, Hestekin JA. Modeling of Clostridium tyrobutyricum for Butyric Acid Selectivity in Continuous Fermentation. Energies. 2014; 7(4):2421-2435.

Chicago/Turabian Style

Du, Jianjun; McGraw, Amy; Hestekin, Jamie A. 2014. "Modeling of Clostridium tyrobutyricum for Butyric Acid Selectivity in Continuous Fermentation." Energies 7, no. 4: 2421-2435.


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