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Energies 2014, 7(12), 7847-7856; doi:10.3390/en7127847

Isolation and Characterization of New Temperature Tolerant Microalgal Strains for Biomass Production

School of Engineering and Science, Jacobs University, 28759 Bremen, Germany
Institute of Environmental Biology and Biotechnology, University of Applied Sciences Bremen, 28199 Bremen, Germany
Author to whom correspondence should be addressed.
Received: 7 August 2014 / Revised: 17 November 2014 / Accepted: 18 November 2014 / Published: 25 November 2014
(This article belongs to the Special Issue Algae Based Technologies)
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Microalgae exhibit great potential for biomass production. Although microalgae display an enormous biodiversity, surprisingly only 15 species are used for large scale production processes worldwide. The implementation of new production strains with good process-oriented properties, especially fast growth rate and heat resistance, could improve production efficiency and reduce costs. In this study 130 environmental samples collected in Germany, Spain, Italy and Portugal were investigated for fast growing thermotolerant photosynthetic species. Isolates were characterized and identified on a molecular level. In total 21 of the isolated freshwater strains were able to grow at 40 °C. Additionally, 13 of those 21 strains are able to grow at 45 °C. The highest growth rate at room temperature was 1.16 per day (isolate T306A), compared to 0.053 per day at 45 °C (isolate Sp13). In three thermotolerant strains pigment production was induced. Molecular identification by 18S rDNA sequencing revealed that the isolates were all chlorophytes belonging to four different families. View Full-Text
Keywords: screening; high temperature tolerance; biomass production; freshwater microalgae; pigments screening; high temperature tolerance; biomass production; freshwater microalgae; pigments

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bleeke, F.; Rwehumbiza, V.M.; Winckelmann, D.; Klöck, G. Isolation and Characterization of New Temperature Tolerant Microalgal Strains for Biomass Production. Energies 2014, 7, 7847-7856.

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