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Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica
Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
* Author to whom correspondence should be addressed.
Received: 21 March 2013; Accepted: 29 March 2013 / Published: 10 April 2013
Abstract: We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.
Keywords: biodiesel; lipase; biomimetic silica; response surface methodology
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Kuan, I.-C.; Lee, C.-C.; Tsai, B.-H.; Lee, S.-L.; Lee, W.-T.; Yu, C.-Y. Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica. Energies 2013, 6, 2052-2064.
Kuan I-C, Lee C-C, Tsai B-H, Lee S-L, Lee W-T, Yu C-Y. Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica. Energies. 2013; 6(4):2052-2064.
Kuan, I-Ching; Lee, Chia-Chi; Tsai, Bing-Hong; Lee, Shiow-Ling; Lee, Wei-Ting; Yu, Chi-Yang. 2013. "Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica." Energies 6, no. 4: 2052-2064.