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Energies 2013, 6(4), 2052-2064; doi:10.3390/en6042052
Article

Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica

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Received: 21 March 2013; Accepted: 29 March 2013 / Published: 10 April 2013
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Abstract: We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.
Keywords: biodiesel; lipase; biomimetic silica; response surface methodology biodiesel; lipase; biomimetic silica; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kuan, I.-C.; Lee, C.-C.; Tsai, B.-H.; Lee, S.-L.; Lee, W.-T.; Yu, C.-Y. Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica. Energies 2013, 6, 2052-2064.

AMA Style

Kuan I-C, Lee C-C, Tsai B-H, Lee S-L, Lee W-T, Yu C-Y. Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica. Energies. 2013; 6(4):2052-2064.

Chicago/Turabian Style

Kuan, I-Ching; Lee, Chia-Chi; Tsai, Bing-Hong; Lee, Shiow-Ling; Lee, Wei-Ting; Yu, Chi-Yang. 2013. "Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica." Energies 6, no. 4: 2052-2064.


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