Energies 2012, 5(11), 4746-4757; doi:10.3390/en5114746
Article

Optimization of Key Factors Affecting Methane Production from Acidic Effluent Coming from the Sugarcane Juice Hydrogen Fermentation Process

Alissara Reungsang 1,2,* email, Sakchai Pattra 3 email and Sureewan Sittijunda 4 email
1 Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand 2 Reserach Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand 3 Community Public Health Program, Faculty of Arts and Science, Chaiyaphum Rajabhat University, Chaiyaphum 36000, Thailand 4 Department of Biotechnology, Faculty of Science, Burapha University, Chonburi 20131, Thailand
* Author to whom correspondence should be addressed.
Received: 27 August 2012; in revised form: 18 October 2012 / Accepted: 8 November 2012 / Published: 19 November 2012
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Abstract: Response surface methodology with a central composite design was applied to optimize the key factors affecting methane production from the acidic effluent coming from the sugarcane juice hydrogen fermentation process. The parameters studied were substrate concentration, ratio of NaHCO3 to substrate concentration and initial pH. The experimental results showed that substrate concentration and initial pH had significant individual (p < 0.05) effect on methane yield (MY). However, there was no interactive effect between these variables (p > 0.05). The maximum MY of 367 mL CH4/g-volatile solid (VS)added was obtained at the optimum conditions of 13,823 mg-COD/L, an NaHCO3 to substrate concentration ratio of 3.09 and an initial pH of 7.07. Under the optimum conditions, MY was enhanced 4.4-fold in comparison to raw effluent.
Keywords: effluent; methane; response surface methodology

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MDPI and ACS Style

Reungsang, A.; Pattra, S.; Sittijunda, S. Optimization of Key Factors Affecting Methane Production from Acidic Effluent Coming from the Sugarcane Juice Hydrogen Fermentation Process. Energies 2012, 5, 4746-4757.

AMA Style

Reungsang A, Pattra S, Sittijunda S. Optimization of Key Factors Affecting Methane Production from Acidic Effluent Coming from the Sugarcane Juice Hydrogen Fermentation Process. Energies. 2012; 5(11):4746-4757.

Chicago/Turabian Style

Reungsang, Alissara; Pattra, Sakchai; Sittijunda, Sureewan. 2012. "Optimization of Key Factors Affecting Methane Production from Acidic Effluent Coming from the Sugarcane Juice Hydrogen Fermentation Process." Energies 5, no. 11: 4746-4757.

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