Biochemical Conversion and Microbial Community in Response to Ternary pH Buffer System during Anaerobic Digestion of Swine Manure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Substrate and Inocula
2.2. Experimental Setup
2.3. DNA Extraction
2.4. Analytical Method
2.4.1. Physicochemical Analyses
2.4.2. Microbial Community Analyses
2.5. Calculations and Statistical Analyses
2.5.1. Extent of Solubilization and Acidification
2.5.2. Efficiency of Biochemical Conversion Data Analysis
3. Results and Discussion
3.1. Overall Performance of AD Reactors
3.1.1. Efficiency of Biochemical Conversion
3.1.2. Evolution of VFA Composition
3.2. Microbial Community Dynamics
3.2.1. Bacterial Community
3.2.2. Archaeal Community
3.3. Relationship with Ternary pH Buffer System
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameters | Units | TS-4 | TS-8 | TS-11 | TS-14 |
---|---|---|---|---|---|
VSin | % | 3.00 ± 0.08 | 6.11 ± 0.03 | 8.32 ± 0.06 | 10.85 ± 0.10 |
VSout | % | 1.08 ± 0.01 | 2.17 ± 0.07 | 3.41 ± 0.11 | 5.70 ± 0.16 |
VS removal | % | 75.6 ± 1.2 | 76.0 ± 1.4 | 69.7 ± 1.9 | 55.8 ± 1.3 |
TS removal | % | 63.0 ± 0.2 | 62.9 ± 0.1 | 56.7 ± 1.2 | 43.9 ± 1.3 |
Cumulative Methane | NmL | 2480 ± 9 | 5741 ± 339 | 6955 ± 593 | 7287 ± 376 |
Methane Potential | NmL/g-VS | 271 ± 1 | 314 ± 19 | 277 ± 24 | 214 ± 11 |
TCOD reduction | % | 74.2 ± 2.9 | 76.7 ± 1.5 | 68.5 ± 3.2 | 65.4 ± 2.8 |
T80a | days | 59 | 104 | 118 | NQ b |
T80*c | days | 56 | 59 | 56 | - |
Samples | pH Value | TAN (mg·L−1) | TAN Increment b | FAN (mg·L−1) | FAN Increment c | Extent of Acidification (%) | Extent of Solubilization (%) | |
---|---|---|---|---|---|---|---|---|
Manure | 7.08 ± 0.08 a | 3974 ± 87 | - | - | - | - | - | |
Inoculum | 8.02 ± 0.04 | 2281 ± 36 | - | 280 ± 4 | - | - | - | |
TS-4 | B0 | 7.21 ± 0.01 | 928 ± 22 | 0 | 20 ± 0 | 0 | 7.35 ± 0.06 | 20.49 ± 0.37 |
B7 | 7.08 ± 0.09 | 1392 ± 62 | 0.50 | 20 ± 0 | 0.00 | 32.52 ± 0.91 | 38.10 ± 0.20 | |
B35 | 7.30 ± 0.04 | 1570 ± 20 | 0.69 | 43 ± 5 | 1.15 | 38.39 ± 0.48 | 45.88 ± 0.06 | |
B63 | 7.79 ± 0.01 | 1608 ± 47 | 0.73 | 125 ± 6 | 5.25 | 38.72 ± 0.61 | 45.92 ± 0.20 | |
B77 | 7.75 ± 0.04 | 1659 ± 86 | 0.79 | 114 ± 15 | 4.70 | 39.59 ± 0.49 | 46.19 ± 1.17 | |
TS-8 | C0 | 6.95 ± 0.01 | 1825 ± 44 | 0 | 22 ± 0 | 0 | 7.54 ± 0.05 | 18.71 ± 1.30 |
C7 | 7.00 ± 0.03 | 3060 ± 158 | 0.68 | 38 ± 0 | 0.73 | 25.45 ± 0.95 | 34.87 ± 0.76 | |
C35 | 6.42 ± 0.01 | 3569 ± 81 | 0.96 | 13 ± 1 | −0.41 | 31.92 ± 1.25 | 39.01 ± 1.45 | |
C77 | 7.73 ± 0.02 | 3232 ± 52 | 0.77 | 223 ± 10 | 9.14 | 32.98 ± 3.27 | 40.18 ± 2.83 | |
C144 | 7.84 ± 0.02 | 3364 ± 47 | 0.84 | 282 ± 4 | 11.82 | 44.10 ± 2.90 | 50.00 ± 1.66 | |
TS-11 | D0 | 6.86 ± 0.00 | 2814 ± 33 | 0 | 27 ± 0 | 0 | 7.58 ± 0.06 | 20.08 ± 0.21 |
D7 | 7.21 ± 0.02 | 4241 ± 93 | 0.51 | 88 ± 2 | 2.26 | 21.71 ± 0.70 | 30.98 ± 1.51 | |
D35 | 6.51 ± 0.06 | 5057 ± 69 | 0.80 | 24 ± 1 | −0.11 | 26.22 ± 1.13 | 34.01 ± 0.77 | |
D77 | 7.99 ± 0.03 | 4887 ± 123 | 0.74 | 588 ± 12 | 20.78 | 28.78 ± 0.77 | 36.37 ± 1.11 | |
D144 | 7.91 ± 0.12 | 4451 ± 123 | 0.58 | 466 ± 98 | 16.26 | 41.10 ± 1.73 | 49.20 ± 0.99 | |
TS-14 | E0 | 6.75 ± 0.02 | 3206 ± 95 | 0 | 24 ± 0 | 0 | 7.83 ± 0.09 | 21.88 ± 0.64 |
E7 | 7.09 ± 0.06 | 5370 ± 405 | 0.67 | 84 ± 16 | 2.50 | 22.57 ± 0.96 | 36.03 ± 1.99 | |
E35 | 6.68 ± 0.04 | 6134 ± 399 | 0.91 | 41 ± 1 | 0.71 | 27.63 ± 1.49 | 38.06 ± 1.60 | |
E77 | 8.09 ± 0.12 | 5918 ± 70 | 0.85 | 734 ± 54 | 29.58 | 31.04 ± 2.43 | 39.51 ± 1.92 | |
E144 | 7.85 ± 0.05 | 5778 ± 0 | 0.80 | 540 ± 32 | 21.50 | 37.19 ± 2.06 | 46.91 ± 1.88 |
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Meng, X.; Zhang, Y.; Sui, Q.; Zhang, J.; Wang, R.; Yu, D.; Wang, Y.; Wei, Y. Biochemical Conversion and Microbial Community in Response to Ternary pH Buffer System during Anaerobic Digestion of Swine Manure. Energies 2018, 11, 2991. https://doi.org/10.3390/en11112991
Meng X, Zhang Y, Sui Q, Zhang J, Wang R, Yu D, Wang Y, Wei Y. Biochemical Conversion and Microbial Community in Response to Ternary pH Buffer System during Anaerobic Digestion of Swine Manure. Energies. 2018; 11(11):2991. https://doi.org/10.3390/en11112991
Chicago/Turabian StyleMeng, Xiaoshan, Yuxiu Zhang, Qianwen Sui, Junya Zhang, Rui Wang, Dawei Yu, Yawei Wang, and Yuansong Wei. 2018. "Biochemical Conversion and Microbial Community in Response to Ternary pH Buffer System during Anaerobic Digestion of Swine Manure" Energies 11, no. 11: 2991. https://doi.org/10.3390/en11112991