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Energies 2017, 10(11), 1696; doi:10.3390/en10111696

The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain

1
School of Life Sciences, Shandong University of Technology, Zibo 255049, China
2
Centre for Marine Bioproducts Development, Department of Medical Biotechnology, Flinders University, Bedford Park, SA 5042, Australia
*
Authors to whom correspondence should be addressed.
Received: 11 September 2017 / Revised: 12 October 2017 / Accepted: 16 October 2017 / Published: 25 October 2017
(This article belongs to the Special Issue Algae Fuel 2017)
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Abstract

In the present study, eight kinds plant growth regulators—salicylic acid (SA), 1-naphthaleneacetic acid (NAA), gibberellic acid (GA3), 6-benzylaminopurine (6-BA), 2, 4-epi-brassinolide (EBR), abscisic acid (ABA), ethephon (ETH), and spermidine (SPD)—were used to investigate the impact on microalgal biomass, lipid, total soluble protein, carotenoids, and polyunsaturated fatty acids (PUFAS) production of Chlorella pyrenoidosa ZF strain. The results showed the quickest biomass enhancement was induced by 50 mg·L−1 NAA, with a 6.3-fold increase over the control; the highest protein content was increased by 0.005 mg·L−1 ETH, which produced 3.5-fold over the control; total carotenoids content was induced most effectively by 1 mg·L−1 NAA with 3.6-fold higher production than the control; the most efficient elicitor for lipid production was 5 mg·L−1 GA3 at 1.9-fold of the control; 0.2 mg·L−1 ETH induced the abundant production of 1.82 ± 0.23% linoleic acid; 0.65 ± 0.01% linolenic acid was induced by 1 mg·L−1 NAA; 2.53 ± 0.15% arachidonic acid and 0.44 ± 0.05% docosahexaenoic acid were induced by 5 mg·L−1 GA3. Transcriptional expression levels of seven lipid-related genes, including ACP, BC, FAD, FATA, KAS, MCTK, and SAD, were studied by real-time RT-q-PCR. 5 mg·L−1 GA3 was the most effective regulator for transcriptional expressions of these seven genes, producing 23-fold ACP, 31-fold BC, 25-fold FAD, 6-fold KAS, 12-fold MCTK compared with the controls, respectively. View Full-Text
Keywords: Chlorella pyrenoidosa; plant growth regulators; secondary metabolites; gene expression; lipid Chlorella pyrenoidosa; plant growth regulators; secondary metabolites; gene expression; lipid
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Du, H.; Ahmed, F.; Lin, B.; Li, Z.; Huang, Y.; Sun, G.; Ding, H.; Wang, C.; Meng, C.; Gao, Z. The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain. Energies 2017, 10, 1696.

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