Next Article in Journal
Wave Equation Datuming Applied to Seismic Data in Shallow Water Environment and Post-Critical Water Bottom Reflection
Next Article in Special Issue
The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain
Previous Article in Journal
Anaerobic Digestion of Feedstock Grown on Marginal Land: Break-Even Electricity Prices
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessReview
Energies 2017, 10(9), 1413; https://doi.org/10.3390/en10091413

Life Cycle Analysis of Endosymbiotic Algae in an Endosymbiotic Situation with Paramecium bursaria Using Capillary Flow Cytometry

Department of Chemical Science and Engineering, National Institute of Technology, Miyakonojo College, Miyazaki 885-8567, Japan
Academic Editor: Paul L. Chen
Received: 10 August 2017 / Revised: 1 September 2017 / Accepted: 6 September 2017 / Published: 15 September 2017
(This article belongs to the Special Issue Algae Fuel 2017)
View Full-Text   |   Download PDF [3495 KB, uploaded 15 September 2017]   |  

Abstract

Along with algae as producers in ecosystems and industrial applications, some microalgae existing in special ecological niches through endosymbiosis with other organisms represent fascinating examples of biological evolution. Although reproducing endosymbiosis experimentally is difficult in many situations, endosymbiosis of several ongoing types is possible. Endosymbiosis in Paramecium bursaria is a particularly excellent model. Although many studies of P. bursaria have specifically examined infection processes such as the host recognition of symbionts, coordination of host-symbiont division, which has been explored for eukaryotic organelles, is worth pursuing. Evaluating the cell (life) cycle of algae is crucially important for algal applications. Flow cytometry (FCM) has been used to study cell cycles of several eukaryotic cells including microalgae. Microscopy, however, has been used mainly to study endosymbiosis, as with P. bursaria, because of their larger size than suitable cells for FCM with hydrodynamic focusing. Vast amounts of time have been expended for microscopic analysis. This review presents an approach using capillary FCM to elucidate the endosymbiosis of P. bursaria. Results reveal that endosymbiotic algae of P. bursaria finely adjust their cell cycle schedule with their comfortable host and show that a coincident endosymbiont–host life cycle is virtually assured in their endosymbiosis. View Full-Text
Keywords: endosymbiosis; microalgae; Chlorella; chlorophyll fluorescence; cell cycle; fluorescence microscopy; evolution endosymbiosis; microalgae; Chlorella; chlorophyll fluorescence; cell cycle; fluorescence microscopy; evolution
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Takahashi, T. Life Cycle Analysis of Endosymbiotic Algae in an Endosymbiotic Situation with Paramecium bursaria Using Capillary Flow Cytometry. Energies 2017, 10, 1413.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Energies EISSN 1996-1073 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top