Resveratrol Inhibited Hydroquinone-Induced Cytotoxicity in Mouse Primary Hepatocytes
Abstract
:1. Introduction
2. Methods
2.1. Chemicals
2.2. Mouse Hepatocyte Isolation and Primary Culture
2.3. Cell Viability Assay
2.4. Measurement of Hydroquinone-Induced Apoptosis
2.5. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
2.6.Statistical Analysis
3. Results and Discussion
3.1. Resveratrol Inhibited Hydroquinone-Induced Apoptosis in Mouse Primary Hepatocytes
3.2. Resveratrol Inhibited Hydroquinone-Induced Cytotoxicity
3.3. Resveratrol Suppressed Hydroquinone-Induced Expression of CYP2E1 mRNA
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Wang, D.-H.; Ootsuki, Y.; Fujita, H.; Miyazaki, M.; Yie, Q.; Tsutsui, K.; Sano, K.; Masuoka, N.; Ogino, K. Resveratrol Inhibited Hydroquinone-Induced Cytotoxicity in Mouse Primary Hepatocytes. Int. J. Environ. Res. Public Health 2012, 9, 3354-3364. https://doi.org/10.3390/ijerph9093354
Wang D-H, Ootsuki Y, Fujita H, Miyazaki M, Yie Q, Tsutsui K, Sano K, Masuoka N, Ogino K. Resveratrol Inhibited Hydroquinone-Induced Cytotoxicity in Mouse Primary Hepatocytes. International Journal of Environmental Research and Public Health. 2012; 9(9):3354-3364. https://doi.org/10.3390/ijerph9093354
Chicago/Turabian StyleWang, Da-Hong, Yoshie Ootsuki, Hirofumi Fujita, Masahiro Miyazaki, Qinxia Yie, Ken Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, and Keiki Ogino. 2012. "Resveratrol Inhibited Hydroquinone-Induced Cytotoxicity in Mouse Primary Hepatocytes" International Journal of Environmental Research and Public Health 9, no. 9: 3354-3364. https://doi.org/10.3390/ijerph9093354