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Int. J. Environ. Res. Public Health 2012, 9(6), 2045-2056; doi:10.3390/ijerph9062045
Article

Molecular Detection and Drug Resistance of Mycobacterium tuberculosis Complex from Cattle at a Dairy Farm in the Nkonkobe Region of South Africa: A Pilot Study

1
, 1,*  and 1,2
Received: 10 April 2012; in revised form: 9 May 2012 / Accepted: 14 May 2012 / Published: 29 May 2012
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Abstract: Mycobacterium tuberculosis complex (MTBC) causes tuberculosis (TB) in humans and animals. We investigated the presence of MTBC in cattle milk and its drug resistance using polymerase chain reaction (PCR). Two hundred samples (100 mL each) were obtained from a dairy farm in the Nkonkobe region of South Africa. The samples were processed using the modified Petroff method. DNA was isolated using a Zymo Bacterial DNA kit and amplified using Seeplex® MTB Nested ACE assay. The Genotype® Mycobacterium tuberculosis-multidrug resistantplus (MTBDRplus) assay was used to perform drug susceptibility and detection of mutations conferring resistance to isoniazid (INH) and rifampicin (RIF). Eleven samples tested positive for MTBC DNA using the Seeplex® MTB Nested ACE assay. The Genotype® MTBDRplus assay showed that 10/11 samples were resistant to both INH and RIF i.e., multi-drug resistant (MDR). The most and least frequent rpoB mutations detected in RIF resistant samples were H526Y (9/10) and D516V (2/10) respectively. None of the INH resistant samples harbored mutations in the katG gene. However, all of them harbored the T8A mutation in the inhA gene. These results have clinical and epidemiological significance and calls for further studies and necessary actions to delineate the situation.
Keywords: Mycobacterium tuberculosis complex; unpasteurized milk; PCR; multidrug resistance; South Africa Mycobacterium tuberculosis complex; unpasteurized milk; PCR; multidrug resistance; South Africa
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Silaigwana, B.; Green, E.; Ndip, R.N. Molecular Detection and Drug Resistance of Mycobacterium tuberculosis Complex from Cattle at a Dairy Farm in the Nkonkobe Region of South Africa: A Pilot Study. Int. J. Environ. Res. Public Health 2012, 9, 2045-2056.

AMA Style

Silaigwana B, Green E, Ndip RN. Molecular Detection and Drug Resistance of Mycobacterium tuberculosis Complex from Cattle at a Dairy Farm in the Nkonkobe Region of South Africa: A Pilot Study. International Journal of Environmental Research and Public Health. 2012; 9(6):2045-2056.

Chicago/Turabian Style

Silaigwana, Blessing; Green, Ezekiel; Ndip, Roland N. 2012. "Molecular Detection and Drug Resistance of Mycobacterium tuberculosis Complex from Cattle at a Dairy Farm in the Nkonkobe Region of South Africa: A Pilot Study." Int. J. Environ. Res. Public Health 9, no. 6: 2045-2056.


Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert