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Fluoro-edenite Fibers Induce Expression of Hsp70 and Inflammatory Response
Department of Physiological Sciences, University of Catania, V.le A. Doria 6, 95125 Catania, Italy
Department of Mineralogical and Petrological Sciences, University of Turin, Turin, Italy, CNR IGG-Sezione I Torino, Italy
Department of Pharmaceutical Sciences, University of Catania, Catania, Italy
Department Biological Chemistry, Clinical Biochemistry and Molecular Biology, University of Catania, Italy
Department of Earth Sciences, University “La Sapienza”, Rome, Italy
Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
* Authors to whom correspondence should be addressed.
Received: 1 June 2007; Accepted: 6 September 2007 / Published: 30 September 2007
Abstract: Many asbestos-like mineral fibers have been detected in the air of mountainous and volcanic areas of Italy and other parts of the world. These fibers have been suspected to be the cause of increased incidences of lung cancer and other lung diseases in these areas. However, the mechanisms of the cellular response and defense following exposure to these microscopic fibers have not been characterized. We continue to study these mechanisms to be able to propose preventive strategies in large populations. The objective of the present study was to determine comparatively biological responses of mesothelial Met-5A and monocyte-macrophage J774 cells following exposure to two types of fluoro-edenite fibers having low and high iron content (labeled 19 and 27, respectively) obtained from Biancavilla (Sicily, Italy). The reference fiber was a non-iron fibrous tremolite from Val di Susa (Piemonte, Italy). The cells were treated with 5, 50, and 100 μg of fibrous matter per 1 ml for 72 hr. We identified several key mechanisms by which cells responded and counteracted the injury induced by these fibers. The fibers caused induction of the heat shock protein 70 (Hsp70), stimulated formation of reactive oxygen species (detected by using DCFH-DA as a fluorescent probe) and NO• (measured as nitrite). Exposure of cells to the fibers induced lactate dehydrogenase activity and decreased viability. The fluoro-endenite type 27 was the most potent fiber tested, which indicated that iron and possibly manganese contribute significantly to this fiber toxicity. The J774 cells were more sensitive to fluoro-edenite than Met-5A cells suggesting that the primary site of the fiberinduced inflammatory response could be the macrophage rather than the pulmonary epithelium. Fluoro-edenite produces more biological alterations with respect to non-iron tremolite. Hsp70 and free radicals could be important factors in the context of mineral fiber-induced acute lung injury leading possibly to mutagenic effects. We anticipate that pharmacological blockade of the fiber-dependent cellular responses could in long term offer preventive approach to combat lung diseases induced by these fibers.
Keywords: asbestos-like fibers; cell cultures; Hsp70; lung disease; oxidative stress; stress protein
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Cardile, V.; Lombardo, L.; Belluso, E.; Panico, A.; Renis, M.; Gianfagna, A.; Balazy, M. Fluoro-edenite Fibers Induce Expression of Hsp70 and Inflammatory Response. Int. J. Environ. Res. Public Health 2007, 4, 195-202.
Cardile V, Lombardo L, Belluso E, Panico A, Renis M, Gianfagna A, Balazy M. Fluoro-edenite Fibers Induce Expression of Hsp70 and Inflammatory Response. International Journal of Environmental Research and Public Health. 2007; 4(3):195-202.
Cardile, Venera; Lombardo, Laura; Belluso, Elena; Panico, Annamaria; Renis, Marcella; Gianfagna, Antonio; Balazy, Michael. 2007. "Fluoro-edenite Fibers Induce Expression of Hsp70 and Inflammatory Response." Int. J. Environ. Res. Public Health 4, no. 3: 195-202.