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Effects of Pristane on Cytochrome P450 Isozyme Expression in Rat Tissues
Breast Cancer Research Laboratory, Department of Biology, and NIH-Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, 1400 Lynch Street, P. O. Box 18540, Jackson, Mississippi, USA
Laboratory for Cancer Research, College of Pharmacy, Rutgers University, Piscataway, NJ 08855-0789, USA
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216-4505, USA
* Author to whom correspondence should be addressed.
Received: 15 November 2004; Accepted: 6 February 2005 / Published: 30 April 2005
Abstract: Chemical carcinogenesis studies are powerful tools to obtain information on potential mechanisms of chemical factors for malignancies. In this study Western blot analyses, using monoclonal antibodies specific for three different cytochrome P450 (CYP) isozymes (CYP1A1, CYP1A2 and CYP2B), were employed to examine the effect(s) of 3-methylcholanthrene and/or pristane (2,6,10,14-tetramethylpentadecane) on the basal and inducible levels of expression of CYP proteins within Copenhagen rat tissues. Pristane exposure led to tissue specific differences in the CYP isozymes expressed and elicited increased CYP protein expression over 3-methylcholanthrene induced levels in microsomes isolated from liver, Peyer's Patches, and thymus. Within the context of the chemical carcinogenesis model employed in this study, these observations correlated with the induction of B-cell malignancies by low doses of 3-methylcholanthrene and of thymic lymphomas by a high 3-methylcholanthrene dose. The data suggest that pristane treatment affects CYP isozyme expression. This pristane-mediated effect clearly could be a contributing factor in the chemical carcinogenesis of the previously observed lymphoid malignancies, and a possible basis for the tumor enhancing effects of pristane.
Keywords: Pristane; Cytochrome P450 (CYP); monoclonal antibodies; 3-methylcholanthrene; phenobarbital; Western blots
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MDPI and ACS Style
Howard, C.B.; Samuel, J.; Henderson, S.B.; Stevens, J.; Thomas, P.E.; Cuchens, M.A. Effects of Pristane on Cytochrome P450 Isozyme Expression in Rat Tissues. Int. J. Environ. Res. Public Health 2005, 2, 138-146.
Howard CB, Samuel J, Henderson SB, Stevens J, Thomas PE, Cuchens MA. Effects of Pristane on Cytochrome P450 Isozyme Expression in Rat Tissues. International Journal of Environmental Research and Public Health. 2005; 2(1):138-146.
Howard, Carolyn B.; Samuel, Jacqueline; Henderson, Shalonda B.; Stevens, Jacqueline; Thomas, Paul E.; Cuchens, Marvin A. 2005. "Effects of Pristane on Cytochrome P450 Isozyme Expression in Rat Tissues." Int. J. Environ. Res. Public Health 2, no. 1: 138-146.