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Int. J. Environ. Res. Public Health 2018, 15(1), 139; doi:10.3390/ijerph15010139

Trypsin Binding with Copper Ions Scavenges Superoxide: Molecular Dynamics-Based Mechanism Investigation

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, Lanzhou University, Lanzhou 730000, China
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Author to whom correspondence should be addressed.
Received: 29 November 2017 / Revised: 27 December 2017 / Accepted: 7 January 2018 / Published: 15 January 2018
(This article belongs to the Special Issue Endocrine Disruptors Leading to Obesity and Related Diseases)
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Trypsin is a serine protease, which has been proved to be a novel superoxide scavenger. The burst of superoxide induced by polychlorinated biphenyls can be impeded by trypsin in both wild type and sod knockout mutants of Escherichia coli. The experimental results demonstrated that the activities of superoxide scavenging of trypsin were significantly accelerated by Cu ions. Also, with the addition of Cu ions, a new β-sheet (β7) transited from a random coil in the Cu(II)-trypsin (TP) system, which was favorable for the formation of more contacts with other sheets of trypsin. Residue–residue network analysis and the porcupine plots proved that the Cu ion in trypsin strengthened some native interactions among residues, which ultimately resulted in much greater stability of the Cu(II)-TP system. Moreover, compact and stable trypsin structures with Cu ions might be responsible for significantly provoking the activity of superoxide scavenging. View Full-Text
Keywords: trypsin; copper ions; superoxide; molecular dynamics; PCBs trypsin; copper ions; superoxide; molecular dynamics; PCBs

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, X.; Zhong, Y.; Zhao, C. Trypsin Binding with Copper Ions Scavenges Superoxide: Molecular Dynamics-Based Mechanism Investigation. Int. J. Environ. Res. Public Health 2018, 15, 139.

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