Association between Promoter Methylation of Gene ERCC3 and Benzene Hematotoxicity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population and Biological Sample Collection
2.2. DNA Methylation Analysis
2.3. Target Prediction of Transcription Factors
2.4. Statistical Analysis
3. Results
3.1. Demographic Characteristics and Benzene Exposure of the Study Population
3.2. Methylation of Gene ERCC3
3.3. Correlation between Hematotoxicity and Aberrant CpG Methylation Induced by Benzene
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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CpG Unit a | In Relation to TSS | Control (n = 24) Mean ± SD (Cm%) | Exposure (n = 76) Mean ± SD (Cm%) | Beta | SE | p Value | FDR Value |
---|---|---|---|---|---|---|---|
1 | −62 bp | 3.04 ± 2.69 | 3.87 ± 3.94 | 0.07 | 0.19 | 0.725 | 0.725 |
2–4 | −43 bp | 4.92 ± 1.53 | 5.86 ± 2.77 | 0.19 | 0.09 | 0.032 * | 0.176 |
5 | −36 bp | 1.38 ± 1.66 | 2.51 ± 3.44 | 0.32 | 0.18 | 0.082 | 0.226 |
6 | −19 bp | 1.21 ± 2.02 | 2.74 ± 3.90 | 0.28 | 0.20 | 0.153 | 0.281 |
7 | −9 bp | 4.54 ± 3.11 | 4.69 ± 4.03 | 0.09 | 0.18 | 0.626 | 0.689 |
9–10 | +30 bp | 6.96 ± 3.13 | 7.74 ± 3.49 | 0.15 | 0.09 | 0.107 | 0.235 |
11 | +56 bp | 1.15 ± 1.68 | 2.81 ± 4.36 | 0.21 | 0.38 | 0.576 | 0.689 |
13–14 | +71 bp | 6.46 ± 4.96 | 7.47 ± 5.50 | 0.19 | 0.19 | 0.317 | 0.436 |
15–16 | +87 bp | 1.54 ± 2.06 | 3.04 ± 3.85 | 0.39 | 0.20 | 0.059 | 0.216 |
17–18 | +99 bp | 6.79 ± 2.50 | 8.45 ± 4.09 | 0.24 | 0.11 | 0.024 * | 0.176 |
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Zheng, M.; Lin, F.; Hou, F.; Li, G.; Zhu, C.; Xu, P.; Xing, C.; Wang, Q. Association between Promoter Methylation of Gene ERCC3 and Benzene Hematotoxicity. Int. J. Environ. Res. Public Health 2017, 14, 921. https://doi.org/10.3390/ijerph14080921
Zheng M, Lin F, Hou F, Li G, Zhu C, Xu P, Xing C, Wang Q. Association between Promoter Methylation of Gene ERCC3 and Benzene Hematotoxicity. International Journal of Environmental Research and Public Health. 2017; 14(8):921. https://doi.org/10.3390/ijerph14080921
Chicago/Turabian StyleZheng, Min, Feiliang Lin, Fenxia Hou, Guilan Li, Caiying Zhu, Peiyu Xu, Caihong Xing, and Qianfei Wang. 2017. "Association between Promoter Methylation of Gene ERCC3 and Benzene Hematotoxicity" International Journal of Environmental Research and Public Health 14, no. 8: 921. https://doi.org/10.3390/ijerph14080921