Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Information
2.2. Synthesis
2.3. Cell Culture
2.4. Treatment of Cells
2.5. ELISA for Angiogenic and Anti-Angiogenic Factors
2.6. Western Blots for VEGFR-1, AT1, and AT2 Receptors
2.7. Statistical Method
3. Results
3.1. PNTU, PFTU, PNTTU, PFTTU, and CuMEPEA Downregulated Angiogenic Factors
3.2. PNTU, PFTU, PNTTU, PFTTU Upregulated Anti-Angiogenic Factors
3.3. PNTU, PFTU, PNTTU, PFTTU, CuMEPEA, TEA Cycle and MACHAM Upregulated AT2 Receptor Expression
3.4. PNTU, PFTU, PNTTU, PFTTU, CuMEPEA, TEA and MACHAM Downregulated VEGFR-1 and AT1 Receptor Expression
3.5. PNTU, PFTU, PNTTU, PFTTU, and CuMEPEA Downregulated AT1
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Pantho, A.F.; Price, M.; Ashraf, A.Z.; Wajid, U.; Khansari, M.E.; Jahan, A.; Afroze, S.H.; Rhaman, M.M.; Johnson, C.R.; Kuehl, T.J.; et al. Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells. Int. J. Environ. Res. Public Health 2017, 14, 517. https://doi.org/10.3390/ijerph14050517
Pantho AF, Price M, Ashraf AZ, Wajid U, Khansari ME, Jahan A, Afroze SH, Rhaman MM, Johnson CR, Kuehl TJ, et al. Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells. International Journal of Environmental Research and Public Health. 2017; 14(5):517. https://doi.org/10.3390/ijerph14050517
Chicago/Turabian StylePantho, Ahmed F., Mason Price, AHM Zuberi Ashraf, Umaima Wajid, Maryam Emami Khansari, Afsana Jahan, Syeda H. Afroze, Md Mhahabubur Rhaman, Corey R. Johnson, Thomas J. Kuehl, and et al. 2017. "Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells" International Journal of Environmental Research and Public Health 14, no. 5: 517. https://doi.org/10.3390/ijerph14050517