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Int. J. Environ. Res. Public Health 2016, 13(10), 993; doi:10.3390/ijerph13100993

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

1
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China
2
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 11 July 2016 / Revised: 20 September 2016 / Accepted: 29 September 2016 / Published: 8 October 2016
View Full-Text   |   Download PDF [3094 KB, uploaded 8 October 2016]   |  

Abstract

There is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nematodes after 48 h of exposure to concentrations of 0.1, 1.0, or 10.0 μg/L. The results demonstrated that epoxiconazole exposure affected spermatogenesis, decreasing the number of total germ cells, mitotic cells, meiotic cells and spermatids, spermatid diameter, and cross-sectional area, and inducing mitotic germ cell proliferation arrest, premature entry into meiosis, and sperm activation inhibition; however, sperm transfer showed no abnormal changes. In addition, the results showed that epoxiconazole activated the transforming growth factor-β (TGFβ) signaling pathway and increased the expression levels of gene daf-1, daf-3, daf-4, daf-5 and daf-7 in nematodes. We therefore propose that epoxiconazole acts by activating the TGFβ signaling pathway, leading to the impairment of spermatogenesis and the consequent decline in male fertility. View Full-Text
Keywords: epoxiconazole; spermatogenesis; TGF-β; Caenorhabditis elegans epoxiconazole; spermatogenesis; TGF-β; Caenorhabditis elegans
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MDPI and ACS Style

Li, Y.; Zhang, M.; Li, S.; Lv, R.; Chen, P.; Liu, R.; Liang, G.; Yin, L. The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis. Int. J. Environ. Res. Public Health 2016, 13, 993.

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