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Int. J. Environ. Res. Public Health 2015, 12(6), 5735-5742; doi:10.3390/ijerph120605735

Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae

1,†
and
2,†,*
1
Henan Postdoctoral Research Base, Food and Bioengineering College, Xuchang University, Xuchang 461000, China
2
Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 28 March 2015 / Accepted: 8 May 2015 / Published: 26 May 2015
View Full-Text   |   Download PDF [661 KB, uploaded 26 May 2015]

Abstract

Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated isothermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as targets, four sets of LAMP primers were designed for identification and differentiation of S. dysgalactiae, S. uberis and S. agalactiae. The detection limit of all four LAMP primer sets were 0.1 pg DNA template per reaction, the LAMP method with 16S rRNA gene and 16S-23S rRNA intergenic spacers as the targets can differentiate the three pathogens, which is potentially useful in epidemiological studies. View Full-Text
Keywords: loop-mediated isothermal amplification (LAMP); 16S rRNA gene; 16S-23S rRNA intergenic spacer; Streptococcus dysgalactiae; Streptococcus uberis; Streptococcus agalactiae loop-mediated isothermal amplification (LAMP); 16S rRNA gene; 16S-23S rRNA intergenic spacer; Streptococcus dysgalactiae; Streptococcus uberis; Streptococcus agalactiae
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, D.; Liu, Y. Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae. Int. J. Environ. Res. Public Health 2015, 12, 5735-5742.

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