Int. J. Environ. Res. Public Health 2013, 10(4), 1304-1311; doi:10.3390/ijerph10041304
Article

Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

Received: 24 January 2013; in revised form: 13 March 2013 / Accepted: 20 March 2013 / Published: 28 March 2013
(This article belongs to the Special Issue Mass Spectrometry and Environmental Analysis)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL−1 PC2 and 100 µg·mL−1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.
Keywords: ion exchange chromatography; mass spectrometry; MALDI-TOF; phytochelatin; cadmium; intramolecular complex
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MDPI and ACS Style

Rodrigo, M.A.M.; Cernei, N.; Kominkova, M.; Zitka, O.; Beklova, M.; Zehnalek, J.; Kizek, R.; Adam, V. Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes. Int. J. Environ. Res. Public Health 2013, 10, 1304-1311.

AMA Style

Rodrigo MAM, Cernei N, Kominkova M, Zitka O, Beklova M, Zehnalek J, Kizek R, Adam V. Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes. International Journal of Environmental Research and Public Health. 2013; 10(4):1304-1311.

Chicago/Turabian Style

Rodrigo, Miguel A.M.; Cernei, Natalia; Kominkova, Marketa; Zitka, Ondrej; Beklova, Miroslava; Zehnalek, Josef; Kizek, Rene; Adam, Vojtech. 2013. "Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes." Int. J. Environ. Res. Public Health 10, no. 4: 1304-1311.

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