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Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Karel Englis College, Sujanovo nam. 356/1, CZ-602 00, Brno, Czech Republic
Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
* Author to whom correspondence should be addressed.
Received: 6 August 2012; in revised form: 6 December 2012 / Accepted: 7 December 2012 / Published: 20 December 2012
Abstract: Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry (antibacterial textiles with silver nanoparticles), electronics (high-resolution imaging, logical circuits on the molecular level) and medicine. Medicine represents one of the most important fields of application of nanomaterials. They are investigated in connection with targeted therapy (infectious diseases, malignant diseases) or imaging (contrast agents). Nanomaterials including nanoparticles have a great application potential in the targeted transport of pharmaceuticals. However, there are some negative properties of nanoparticles, which must be carefully solved, as hydrophobic properties leading to instability in aqueous environment, and especially their possible toxicity. Data about toxicity of nanomaterials are still scarce. Due to this fact, in this work we focused on studying of the effect of magnetic nanoparticles (NPs) and modified magnetic nanoparticles (MNPs) on tobacco BY-2 plant cell suspension culture. We aimed at examining the effect of NPs and MNPs on growth, proteosynthesis — total protein content, thiols — reduced (GSH) and oxidized (GSSG) glutathione, phytochelatins PC2-5, glutathione S-transferase (GST) activity and antioxidant activity of BY-2 cells. Whereas the effect of NPs and MNPs on growth of cell suspension culture was only moderate, significant changes were detected in all other biochemical parameters. Significant changes in protein content, phytochelatins levels and GST activity were observed in BY-2 cells treated with MNPs nanoparticles treatment. Changes were also clearly evident in the case of application of NPs. Our results demonstrate the ability of MNPs to negatively affect metabolism and induce biosynthesis of protective compounds in a plant cell model represented by BY-2 cell suspension culture. The obtained results are discussed, especially in connection with already published data. Possible mechanisms of NPs’ and MNPs’ toxicity are introduced.
Keywords: nanoparticles; plant cell; thiol compounds; glutathione; phytochelatin
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Krystofova, O.; Sochor, J.; Zitka, O.; Babula, P.; Kudrle, V.; Adam, V.; Kizek, R. Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture. Int. J. Environ. Res. Public Health 2013, 10, 47-71.
Krystofova O, Sochor J, Zitka O, Babula P, Kudrle V, Adam V, Kizek R. Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture. International Journal of Environmental Research and Public Health. 2013; 10(1):47-71.
Krystofova, Olga; Sochor, Jiri; Zitka, Ondrej; Babula, Petr; Kudrle, Vit; Adam, Vojtech; Kizek, Rene. 2013. "Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture." Int. J. Environ. Res. Public Health 10, no. 1: 47-71.