Abstract: Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors.
Keywords: 13-acetoxysarcocrassolide; Sarcophyton crassocaule; BFTC cells; proteomic analysis
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Su, C.-C.; Su, J.-H.; Lin, J.-J.; Chen, C.-C.; Hwang, W.-I.; Huang, H.H.; Wu, Y.-J. An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells. Mar. Drugs 2011, 9, 2622-2642.
Su C-C, Su J-H, Lin J-J, Chen C-C, Hwang W-I, Huang HH, Wu Y-J. An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells. Marine Drugs. 2011; 9(12):2622-2642.
Su, Ching-Chyuan; Su, Jui-Hsin; Lin, Jen-Jie; Chen, Cheng-Chi; Hwang, Wen-Ing; Huang, Han Hsiang; Wu, Yu-Jen. 2011. "An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells." Mar. Drugs 9, no. 12: 2622-2642.