Mar. Drugs 2011, 9(11), 2236-2255; doi:10.3390/md9112236
Article

Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure

CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal
* Author to whom correspondence should be addressed.
Received: 22 September 2011; in revised form: 21 October 2011 / Accepted: 28 October 2011 / Published: 7 November 2011
(This article belongs to the Special Issue Marine Anti-infective Agents)
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Abstract: Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by the soft agar overlay technique. The host specificity was evaluated by cross infection. The ecological impact of phage addition on the structure of the bacterial community was evaluated by DGGE of PCR amplified 16S rRNA gene fragments. The survival period varied between 12 and 91 days, with a higher viability for Aeromonas salmonicida phages. The phages of Vibrio parahaemolyticus and of A. salmonicida infected bacteria of different families with a high efficacy of plating. The specific phages of pathogenic bacteria had no detectable impact on the structure of the bacterial community. In conclusion, V. parahaemolyticus and A. salmonicida phages show good survival time in marine water, have only a moderated impact on the overall bacterial community structure and the desired specificity for host pathogenic bacteria, being potential candidates for therapy of fish infectious diseases in marine aquaculture systems.
Keywords: phage therapy; bacteriophages; fish pathogenic bacteria; aquaculture; bacterial community structure

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MDPI and ACS Style

Pereira, C.; Silva, Y.J.; Santos, A.L.; Cunha, Â.; Gomes, N.C.M.; Almeida, A. Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure. Mar. Drugs 2011, 9, 2236-2255.

AMA Style

Pereira C, Silva YJ, Santos AL, Cunha Â, Gomes NCM, Almeida A. Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure. Marine Drugs. 2011; 9(11):2236-2255.

Chicago/Turabian Style

Pereira, Carla; Silva, Yolanda J.; Santos, Ana L.; Cunha, Ângela; Gomes, Newton C. M.; Almeida, Adelaide. 2011. "Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure." Mar. Drugs 9, no. 11: 2236-2255.

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