Next Article in Journal
Previous Article in Journal
Mar. Drugs 2010, 8(10), 2605-2618; doi:10.3390/md8102605
Article

Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-α and IL-6 in LPS-Induced Macrophages

1
, 2
, 1,3
, 1
, 1
, 4
, 3,5
, 1
 and 1,*
Received: 28 August 2010; in revised form: 23 September 2010 / Accepted: 29 September 2010 / Published: 11 October 2010
View Full-Text   |   Download PDF [281 KB, uploaded 11 October 2010]   |   Browse Figures
Abstract: A rapid method for characterization and online detection of surfactin isomers was developed based on HPLC-MSn (n = 1, 2, 3) analyses, and many surfactin isomers were detected and characterized from the bioactive fraction of the mangrove bacterium Bacillus sp. Inhibitory activities of surfactin isomers on the overproduction of nitric oxide and the release of TNF-a and IL-6 in LPS-induced macrophages were systematically investigated. It was revealed that the surfactin isomers showed strong inhibitory properties on the overproduction of nitric oxide and the release of IL-6 on LPS-induced murine macrophage cell RAW264.7 with IC50 values ranging from 1.0 to 7.0 mM. Structure-activity relationship (SAR) studies revealed that the existence of the free carboxyl group in the structure of surfactin isomers was crucial. These findings will be very helpful for the development of this novel kind of natural product as new anti-inflammatory agents.
Keywords: LC-MS; Bacillus sp.; surfactin isomer; anti-inflammatory activity LC-MS; Bacillus sp.; surfactin isomer; anti-inflammatory activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Tang, J.-S.; Zhao, F.; Gao, H.; Dai, Y.; Yao, Z.-H.; Hong, K.; Li, J.; Ye, W.-C.; Yao, X.-S. Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-α and IL-6 in LPS-Induced Macrophages. Mar. Drugs 2010, 8, 2605-2618.

AMA Style

Tang J-S, Zhao F, Gao H, Dai Y, Yao Z-H, Hong K, Li J, Ye W-C, Yao X-S. Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-α and IL-6 in LPS-Induced Macrophages. Marine Drugs. 2010; 8(10):2605-2618.

Chicago/Turabian Style

Tang, Jin-Shan; Zhao, Feng; Gao, Hao; Dai, Yi; Yao, Zhi-Hong; Hong, Kui; Li, Jia; Ye, Wen-Cai; Yao, Xin-Sheng. 2010. "Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-α and IL-6 in LPS-Induced Macrophages." Mar. Drugs 8, no. 10: 2605-2618.



Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert