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A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation
Department of Biotechnology, School of Environmental and Chemical Engineering, Dalian Jiaotong University, 116028 Dalian, China
* Author to whom correspondence should be addressed.
Received: 27 May 2009; in revised form: 17 July 2009 / Accepted: 20 July 2009 / Published: 21 July 2009
Abstract: A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 °C to 30 °C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized.
Keywords: marine sulfate-reducing bacterium; isolation; identification; broad-spectrum antimicrobial activity; mono-n-butyl phthalate
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Zhang, Y.; Mu, J.; Gu, X.; Zhao, C.; Wang, X.; Xie, Z. A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation. Mar. Drugs 2009, 7, 341-354.
Zhang Y, Mu J, Gu X, Zhao C, Wang X, Xie Z. A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation. Marine Drugs. 2009; 7(3):341-354.
Zhang, Yi; Mu, Jun; Gu, Xiaojie; Zhao, Chenyan; Wang, Xiaoliang; Xie, Zeping. 2009. "A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation." Mar. Drugs 7, no. 3: 341-354.