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Mar. Drugs 2008, 6(3), 418-430; doi:10.3390/md6030418
Article

Purification, Characterization and in vitro Anti-Tumor Activity of Proteins from Arca subcrenata Lischke

1,2, 2, 2,* , 2, 2 and 1,*
1 College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, P.R. China 2 College of Pharmacy, Jinan University, Guangzhou 510632, P.R. China
* Authors to whom correspondence should be addressed.
Received: 2 February 2008 / Revised: 8 April 2008 / Accepted: 26 June 2008 / Published: 10 July 2008
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Abstract

Two purified proteins G-6 and G-4-2 were obtained from Arca subcrenata Lischke using the homogenization, salting-out with ammonium sulfate, ion-exchange chromatography and gel filtration chromatography techniques. The purity of G-6 and G-4-2 was over 96%, as measured by RP-HPLC. G-6 and G-4-2 were measured by SDS-PAGE and IEF-PAGE to have molecular weights of 8.2 kDa and 16.0 kDa, and isoelectric points of 6.6 and 6.1, respectively. The amino acid constituents of G-6 and G-4-2 were also determined. The existence of saccharides in G-6 was demonstrated by the phenol-sulfuric acid method. G-6 and G-4-2 inhibited the proliferation of human tumor cells in vitro. By MTT assay, the IC50 values of G-4-2 were 22.9 μg/mL, 46.1 μg/mL and 57.7 μg/mL against Hela, HL-60 and KB cell lines, respectively, and the IC50 value of G-6 against HL-60 cell line was measured to be 123.2 μg/mL.
Keywords: Arca subcrenata Lischke; protein; purification; in vitro anti-tumor activity Arca subcrenata Lischke; protein; purification; in vitro anti-tumor activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Song, L.; Ren, S.; Yu, R.; Yan, C.; Li, T.; Zhao, Y. Purification, Characterization and in vitro Anti-Tumor Activity of Proteins from Arca subcrenata Lischke. Mar. Drugs 2008, 6, 418-430.

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