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Mar. Drugs 2008, 6(3), 418-430; doi:10.3390/md6030418
Article

Purification, Characterization and in vitro Anti-Tumor Activity of Proteins from Arca subcrenata Lischke

1,2, 2, 2,* , 2, 2 and 1,*
1 College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, P.R. China 2 College of Pharmacy, Jinan University, Guangzhou 510632, P.R. China
* Authors to whom correspondence should be addressed.
Received: 2 February 2008 / Revised: 8 April 2008 / Accepted: 26 June 2008 / Published: 10 July 2008
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Abstract

Two purified proteins G-6 and G-4-2 were obtained from Arca subcrenata Lischke using the homogenization, salting-out with ammonium sulfate, ion-exchange chromatography and gel filtration chromatography techniques. The purity of G-6 and G-4-2 was over 96%, as measured by RP-HPLC. G-6 and G-4-2 were measured by SDS-PAGE and IEF-PAGE to have molecular weights of 8.2 kDa and 16.0 kDa, and isoelectric points of 6.6 and 6.1, respectively. The amino acid constituents of G-6 and G-4-2 were also determined. The existence of saccharides in G-6 was demonstrated by the phenol-sulfuric acid method. G-6 and G-4-2 inhibited the proliferation of human tumor cells in vitro. By MTT assay, the IC50 values of G-4-2 were 22.9 μg/mL, 46.1 μg/mL and 57.7 μg/mL against Hela, HL-60 and KB cell lines, respectively, and the IC50 value of G-6 against HL-60 cell line was measured to be 123.2 μg/mL.
Keywords: Arca subcrenata Lischke; protein; purification; in vitro anti-tumor activity Arca subcrenata Lischke; protein; purification; in vitro anti-tumor activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Song, L.; Ren, S.; Yu, R.; Yan, C.; Li, T.; Zhao, Y. Purification, Characterization and in vitro Anti-Tumor Activity of Proteins from Arca subcrenata Lischke. Mar. Drugs 2008, 6, 418-430.

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