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Chemopreventive Effects of Sarcotriol on Ultraviolet B-induced Skin Tumor Development in SKH-1 Hairless Mice
Department of Pharmaceutical Sciences, South Dakota State University, Box 2202C, Brookings, SD 57007, USA
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
* Author to whom correspondence should be addressed.
Received: 2 October 2007 / Accepted: 11 December 2007 / Published: 12 December 2007
Abstract: Sarcotriol (ST) has been shown to be chemopreventive on 7,12-dimethylbenz( a)anthracene (DMBA) initiated and 12-O-tetradecanoylphorbol-13-acetate (TPA)- promoted skin tumor development in CD-1 mice in recent studies from our laboratory. The objective of this study was to determine the chemopreventive effects of ST on ultraviolet B (UVB)-induced skin tumor development in female SKH-1 hairless mice, an experimental model relevant to human skin cancer development, and its possible mechanisms of action. Female SKH-1 mice were divided into two groups: Control and ST treated. Control was topically treated with 100 μL acetone and ST treated group administered with 30 μg ST in 100 μL acetone one hour before UVB exposure. For UVB-induced tumorigenesis, carcinogenesis was initiated and promoted by UVB (180 mJ/cm2). Group weights and tumor counts were taken once every week. After 30 weeks, mice were sacrificed and dorsal skin samples were collected. The proteins from the skin sample were further used for SDSPAGE and Western blotting using specific antibodies against caspase-3, caspase-8, caspase-9 and p53. Tumor multiplicity was found 19.6, 5.2 in the control and ST treated groups respectively. Caspase-3, -8, -9 and p53 were significantly (P < 0.05) upregulated in ST treated group compared to Control group. Together, this study for the first time identifies the chemopreventive effects of ST in UVB-induced carcinogenesis possibly by inducing apoptosis and upregulating p53.
Keywords: Cancer chemoprevention; Skin cancer; Cembranoids; Sarcophine; Sarcophyton glaucum; Sarcotriol; Apoptosis; p53
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MDPI and ACS Style
Kundoor, V.; Zhang, X.; Bommareddy, A.; Khalifa, S.; Fahmy, H.; Dwivedi, C. Chemopreventive Effects of Sarcotriol on Ultraviolet B-induced Skin Tumor Development in SKH-1 Hairless Mice. Mar. Drugs 2007, 5, 197-207.
Kundoor V, Zhang X, Bommareddy A, Khalifa S, Fahmy H, Dwivedi C. Chemopreventive Effects of Sarcotriol on Ultraviolet B-induced Skin Tumor Development in SKH-1 Hairless Mice. Marine Drugs. 2007; 5(4):197-207.
Kundoor, Vipra; Zhang, Xiaoying; Bommareddy, Ajay; Khalifa, Sherief; Fahmy, Hesham; Dwivedi, Chandradhar. 2007. "Chemopreventive Effects of Sarcotriol on Ultraviolet B-induced Skin Tumor Development in SKH-1 Hairless Mice." Mar. Drugs 5, no. 4: 197-207.