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Mar. Drugs 2018, 16(9), 318; https://doi.org/10.3390/md16090318

The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model

1
Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Avenida Salgado Filho, BR 101, Campus Universitario, Lagoa Nova, Natal, RN 59078-900, Brazil
2
Laboratory of Marine Algae, Department of Systematics and Ecology, Federal University of Paraiba, Joao Pessoa, PB 58051-900, Brazil
3
Laboratory of Animal Experimentation of the Institute of Immunobiological Technology (LAEAN/Bio-Manguinhos), Rio de Janeiro, RJ 21040-900, Brazil
4
School of Nursing and Pharmacy, Federal University of Alagoas, Maceió, AL 57072-900, Brazil
5
Academic Unit of Biological Sciences, Federal University of Campina Grande, Patos, PB 58708-110, Brazil
6
Department of Cell Biology and Genetics, Federal University of Rio Grande do Norte, Avenida Salgado Filho, BR 101, Campus Universitario, Lagoa Nova, Natal, RN 59078-900, Brazil
7
Postgraduate Program in Natural Products and Synthetic Bioactive and Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil
*
Author to whom correspondence should be addressed.
Received: 21 July 2018 / Revised: 27 August 2018 / Accepted: 27 August 2018 / Published: 7 September 2018
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Abstract

Caulerpin (CLP), an alkaloid from algae of the genus Caulerpa, has shown anti-inflammatory activity. Therefore, this study aimed to analyze the effect of CLP in the murine model of peritonitis and ulcerative colitis. Firstly, the mice were submitted to peritonitis to evaluate which dose of CLP (40, 4, or 0.4 mg/kg) could decrease the inflammatory infiltration in the peritoneum. The most effective doses were 40 and 4 mg/kg. Then, C57BL/6 mice were submitted to colitis development with 3% dextran sulfate sodium (DSS) and treated with CLP at doses of 40 and 4 mg/kg. The disease development was analyzed through the disease activity index (DAI); furthermore, colonic tissue samples were submitted to histological analysis, NFκB determination, and in vitro culture for cytokines assay. Therefore, CLP at 4 mg/kg presented the best results, triggering improvement of DAI and attenuating the colon shortening and damage. This dose was able to reduce the TNF-α, IFN-γ, IL-6, IL-17, and NFκB p65 levels, and increased the levels of IL-10 in the colon tissue. Thus, CLP mice treatment at a dose of 4 mg/kg showed promising results in ameliorating the damage observed in the ulcerative colitis. View Full-Text
Keywords: caulerpin; ulcerative colitis; anti-inflammatory activity; cytokines; tissue damage caulerpin; ulcerative colitis; anti-inflammatory activity; cytokines; tissue damage
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Lucena, A.M.M.; Souza, C.R.M.; Jales, J.T.; Guedes, P.M.M.; de Miranda, G.E.C.; de Moura, A.M.A.; Araújo-Júnior, J.X.; Nascimento, G.J.; Scortecci, K.C.; Santos, B.V.O.; Souto, J.T. The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model. Mar. Drugs 2018, 16, 318.

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