Next Article in Journal
Fucoidan Does Not Exert Anti-Tumorigenic Effects on Uveal Melanoma Cell Lines
Previous Article in Journal
Asperentin B, a New Inhibitor of the Protein Tyrosine Phosphatase 1B
Previous Article in Special Issue
The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Mar. Drugs 2017, 15(6), 189; doi:10.3390/md15060189

Development of a Dunaliella tertiolecta Strain with Increased Zeaxanthin Content Using Random Mutagenesis

1
Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea
2
Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Adele Cutignano and Giovanna Romano
Received: 20 February 2017 / Revised: 12 June 2017 / Accepted: 18 June 2017 / Published: 21 June 2017
(This article belongs to the Special Issue Marine Microalgae)
View Full-Text   |   Download PDF [2735 KB, uploaded 21 June 2017]   |  

Abstract

Zeaxanthin is a xanthophyll pigment that is regarded as one of the best carotenoids for the prevention and treatment of degenerative diseases. In the worldwide natural products market, consumers prefer pigments that have been produced from biological sources. In this study, a Dunaliella tertiolecta strain that has 10–15% higher cellular zeaxanthin content than the parent strain (zea1), was obtained by random mutagenesis using ethyl methanesulfonate (EMS) as a mutagen. This mutant, mp3, was grown under various salinities and light intensities to optimize culture conditions for zeaxanthin production. The highest cellular zeaxanthin content was observed at 1.5 M NaCl and 65–85 μmol photons·m−2·s−1, and the highest daily zeaxanthin productivity was observed at 0.6 M NaCl and 140–160 μmol photons·m−2·s−1. The maximal yield of zeaxanthin from mp3 in fed-batch culture was 8 mg·L−1, which was obtained at 0.6 M NaCl and 140–160 μmol photons·m−2·s−1. These results suggest that random mutagenesis with EMS is useful for generating D. tertiolecta strains with increased zeaxanthin content, and also suggest optimal culture conditions for the enhancement of biomass and zeaxanthin production by the zeaxanthin accumulating mutant strains. View Full-Text
Keywords: zeaxanthin; Dunaliella tertiolecta; EMS mutagenesis; salinity; light intensity; repeated fed-batch culture zeaxanthin; Dunaliella tertiolecta; EMS mutagenesis; salinity; light intensity; repeated fed-batch culture
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kim, M.; Ahn, J.; Jeon, H.; Jin, E. Development of a Dunaliella tertiolecta Strain with Increased Zeaxanthin Content Using Random Mutagenesis. Mar. Drugs 2017, 15, 189.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top