Interpreting Microbial Biosynthesis in the Genomic Age: Biological and Practical Considerations
AbstractGenome mining has become an increasingly powerful, scalable, and economically accessible tool for the study of natural product biosynthesis and drug discovery. However, there remain important biological and practical problems that can complicate or obscure biosynthetic analysis in genomic and metagenomic sequencing projects. Here, we focus on limitations of available technology as well as computational and experimental strategies to overcome them. We review the unique challenges and approaches in the study of symbiotic and uncultured systems, as well as those associated with biosynthetic gene cluster (BGC) assembly and product prediction. Finally, to explore sequencing parameters that affect the recovery and contiguity of large and repetitive BGCs assembled de novo, we simulate Illumina and PacBio sequencing of the Salinispora tropica genome focusing on assembly of the salinilactam (slm) BGC. View Full-Text
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Miller, I.J.; Chevrette, M.G.; Kwan, J.C. Interpreting Microbial Biosynthesis in the Genomic Age: Biological and Practical Considerations. Mar. Drugs 2017, 15, 165.
Miller IJ, Chevrette MG, Kwan JC. Interpreting Microbial Biosynthesis in the Genomic Age: Biological and Practical Considerations. Marine Drugs. 2017; 15(6):165.Chicago/Turabian Style
Miller, Ian J.; Chevrette, Marc G.; Kwan, Jason C. 2017. "Interpreting Microbial Biosynthesis in the Genomic Age: Biological and Practical Considerations." Mar. Drugs 15, no. 6: 165.
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