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Mar. Drugs 2017, 15(2), 29; doi:10.3390/md15020029

Separation and Characterization of Angiotensin I Converting Enzyme (ACE) Inhibitory Peptides from Saurida elongata Proteins Hydrolysate by IMAC-Ni2+

Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China
Medical College, Guangxi University of Science and Technology, Liuzhou 545006, China
College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China
Authors to whom correspondence should be addressed.
Academic Editor: Se-Kwon Kim
Received: 28 October 2016 / Revised: 18 January 2017 / Accepted: 25 January 2017 / Published: 15 February 2017
View Full-Text   |   Download PDF [2188 KB, uploaded 15 February 2017]   |  


Lizard fish protein hydrolysates (LFPH) were prepared from Lizard fish (Saurida elongata) proteins possessing powerful angiotensin I converting enzyme (ACE) inhibitory activity and the fraction (LFPH-I) with high ACE inhibitory activity was obtained through ultrafiltration. The active Fraction (F2) was isolated from LFPH-I using immobilized metal affinity chromatography (IMAC-Ni2+). Analysis of amino acid levels revealed that F2 eluted from IMAC was enriched in Met, His, Tyr, Pro, Ile, and Leu compared to the crude peptide LFPH-I. F2 with the high ACE inhibitory activity (IC50 of 0.116 mg·mL−1) was further separated by a reverse-phase column to yield a novel ACE inhibitory peptide with IC50 value of 52 μM. The ACE inhibitory peptide was identified as Arg-Tyr-Arg-Pro, RYRP. The present study demonstrated that IMAC may be a useful tool for the separation of ACE inhibitory peptides from protein hydrolysate. View Full-Text
Keywords: ACE-inhibitory activity; lizard fish; hydrolysate; purification; IMAC ACE-inhibitory activity; lizard fish; hydrolysate; purification; IMAC

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Sun, L.; Wu, S.; Zhou, L.; Wang, F.; Lan, X.; Sun, J.; Tong, Z.; Liao, D. Separation and Characterization of Angiotensin I Converting Enzyme (ACE) Inhibitory Peptides from Saurida elongata Proteins Hydrolysate by IMAC-Ni2+. Mar. Drugs 2017, 15, 29.

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