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Mar. Drugs 2017, 15(2), 29; doi:10.3390/md15020029

Separation and Characterization of Angiotensin I Converting Enzyme (ACE) Inhibitory Peptides from Saurida elongata Proteins Hydrolysate by IMAC-Ni2+

1
Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China
2
Medical College, Guangxi University of Science and Technology, Liuzhou 545006, China
3
College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Se-Kwon Kim
Received: 28 October 2016 / Revised: 18 January 2017 / Accepted: 25 January 2017 / Published: 15 February 2017
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Abstract

Lizard fish protein hydrolysates (LFPH) were prepared from Lizard fish (Saurida elongata) proteins possessing powerful angiotensin I converting enzyme (ACE) inhibitory activity and the fraction (LFPH-I) with high ACE inhibitory activity was obtained through ultrafiltration. The active Fraction (F2) was isolated from LFPH-I using immobilized metal affinity chromatography (IMAC-Ni2+). Analysis of amino acid levels revealed that F2 eluted from IMAC was enriched in Met, His, Tyr, Pro, Ile, and Leu compared to the crude peptide LFPH-I. F2 with the high ACE inhibitory activity (IC50 of 0.116 mg·mL−1) was further separated by a reverse-phase column to yield a novel ACE inhibitory peptide with IC50 value of 52 μM. The ACE inhibitory peptide was identified as Arg-Tyr-Arg-Pro, RYRP. The present study demonstrated that IMAC may be a useful tool for the separation of ACE inhibitory peptides from protein hydrolysate. View Full-Text
Keywords: ACE-inhibitory activity; lizard fish; hydrolysate; purification; IMAC ACE-inhibitory activity; lizard fish; hydrolysate; purification; IMAC
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Sun, L.; Wu, S.; Zhou, L.; Wang, F.; Lan, X.; Sun, J.; Tong, Z.; Liao, D. Separation and Characterization of Angiotensin I Converting Enzyme (ACE) Inhibitory Peptides from Saurida elongata Proteins Hydrolysate by IMAC-Ni2+. Mar. Drugs 2017, 15, 29.

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