Sequencing and Characterization of Novel PII Signaling Protein Gene in Microalga Haematococcus pluvialis
AbstractThe PII signaling protein is a key protein for controlling nitrogen assimilatory reactions in most organisms, but little information is reported on PII proteins of green microalga Haematococcus pluvialis. Since H. pluvialis cells can produce a large amount of astaxanthin upon nitrogen starvation, its PII protein may represent an important factor on elevated production of Haematococcus astaxanthin. This study identified and isolated the coding gene (HpGLB1) from this microalga. The full-length of HpGLB1 was 1222 bp, including 621 bp coding sequence (CDS), 103 bp 5′ untranslated region (5′ UTR), and 498 bp 3′ untranslated region (3′ UTR). The CDS could encode a protein with 206 amino acids (HpPII). Its calculated molecular weight (Mw) was 22.4 kDa and the theoretical isoelectric point was 9.53. When H. pluvialis cells were exposed to nitrogen starvation, the HpGLB1 expression was increased 2.46 times in 48 h, concomitant with the raise of astaxanthin content. This study also used phylogenetic analysis to prove that HpPII was homogeneous to the PII proteins of other green microalgae. The results formed a fundamental basis for the future study on HpPII, for its potential physiological function in Haematococcus astaxanthin biosysthesis. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Ma, R.; Li, Y.; Lu, Y. Sequencing and Characterization of Novel PII Signaling Protein Gene in Microalga Haematococcus pluvialis. Mar. Drugs 2017, 15, 304.
Ma R, Li Y, Lu Y. Sequencing and Characterization of Novel PII Signaling Protein Gene in Microalga Haematococcus pluvialis. Marine Drugs. 2017; 15(10):304.Chicago/Turabian Style
Ma, Ruijuan; Li, Yan; Lu, Yinghua. 2017. "Sequencing and Characterization of Novel PII Signaling Protein Gene in Microalga Haematococcus pluvialis." Mar. Drugs 15, no. 10: 304.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.