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Mar. Drugs 2017, 15(10), 296; doi:10.3390/md15100296

Metabolic Engineering of Escherichia coli for Producing Astaxanthin as the Predominant Carotenoid

Institute of Synthetic Biology, Biomedical Center, Guangdong Province Key Laboratory for Aquatic Economic Animals and South China Sea Bio-Resource Exploitation, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 4 August 2017 / Revised: 17 September 2017 / Accepted: 20 September 2017 / Published: 22 September 2017
(This article belongs to the Collection Marine Carotenoids)
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Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. A higher ratio of astaxanthin to the total carotenoids is required for efficient astaxanthin production. β-Carotene ketolase and hydroxylase play important roles in astaxanthin production. We first compared the conversion efficiency to astaxanthin in several β-carotene ketolases from Brevundimonas sp. SD212, Sphingomonas sp. DC18, Paracoccus sp. PC1, P. sp. N81106 and Chlamydomonas reinhardtii with the recombinant Escherichia coli cells that synthesize zeaxanthin due to the presence of the Pantoea ananatis crtEBIYZ. The B. sp. SD212 crtW and P. ananatis crtZ genes are the best combination for astaxanthin production. After balancing the activities of β-carotene ketolase and hydroxylase, an E. coli ASTA-1 that carries neither a plasmid nor an antibiotic marker was constructed to produce astaxanthin as the predominant carotenoid (96.6%) with a specific content of 7.4 ± 0.3 mg/g DCW without an addition of inducer. View Full-Text
Keywords: astaxanthin; Escherichia coli; metabolic engineering; β-carotene ketolase; β-carotene hydroxylase astaxanthin; Escherichia coli; metabolic engineering; β-carotene ketolase; β-carotene hydroxylase

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Lu, Q.; Bu, Y.-F.; Liu, J.-Z. Metabolic Engineering of Escherichia coli for Producing Astaxanthin as the Predominant Carotenoid. Mar. Drugs 2017, 15, 296.

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