Next Article in Journal
New Cyclotetrapeptides and a New Diketopiperzine Derivative from the Marine Sponge-Associated Fungus Neosartorya glabra KUFA 0702
Next Article in Special Issue
The Role of Spongia sp. in the Discovery of Marine Lead Compounds
Previous Article in Journal
New Polyketides and New Benzoic Acid Derivatives from the Marine Sponge-Associated Fungus Neosartorya quadricincta KUFA 0081
Previous Article in Special Issue
Guanidine Alkaloids from the Marine Sponge Monanchora pulchra Show Cytotoxic Properties and Prevent EGF-Induced Neoplastic Transformation in Vitro
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2016, 14(7), 135; doi:10.3390/md14070135

Transcriptome of the Australian Mollusc Dicathais orbita Provides Insights into the Biosynthesis of Indoles and Choline Esters

1
Southern Cross Plant Science, Southern Cross University, Lismore NSW 2480, Australia
2
Marine Ecology Research Centre, School of Environment, Science and Engineering, Southern Cross University, Lismore NSW 2480, Australia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Long
Received: 16 May 2016 / Revised: 8 July 2016 / Accepted: 12 July 2016 / Published: 20 July 2016
(This article belongs to the Collection Bioactive Compounds from Marine Invertebrates)
View Full-Text   |   Download PDF [4343 KB, uploaded 20 July 2016]   |  

Abstract

Dicathais orbita is a mollusc of the Muricidae family and is well known for the production of the expensive dye Tyrian purple and its brominated precursors that have anticancer properties, in addition to choline esters with muscle-relaxing properties. However, the biosynthetic pathways that produce these secondary metabolites in D. orbita are not known. Illumina HiSeq 2000 transcriptome sequencing of hypobranchial glands, prostate glands, albumen glands, capsule glands, and mantle and foot tissues of D. orbita generated over 201 million high quality reads that were de novo assembled into 219,437 contigs. Annotation with reference to the Nr, Swiss-Prot and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases identified candidate-coding regions in 76,152 of these contigs, with transcripts for many enzymes in various metabolic pathways associated with secondary metabolite biosynthesis represented. This study revealed that D. orbita expresses a number of genes associated with indole, sulfur and histidine metabolism pathways that are relevant to Tyrian purple precursor biosynthesis, and many of which were not found in the fully annotated genomes of three other molluscs in the KEGG database. However, there were no matches to known bromoperoxidase enzymes within the D. orbita transcripts. These transcriptome data provide a significant molecular resource for gastropod research in general and Tyrian purple producing Muricidae in particular. View Full-Text
Keywords: Muricidae; transcriptomics; Tyrian purple; tryptophan; metabolic pathways Muricidae; transcriptomics; Tyrian purple; tryptophan; metabolic pathways
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Baten, A.; Ngangbam, A.K.; Waters, D.L.E.; Benkendorff, K. Transcriptome of the Australian Mollusc Dicathais orbita Provides Insights into the Biosynthesis of Indoles and Choline Esters. Mar. Drugs 2016, 14, 135.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top