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Mar. Drugs 2016, 14(6), 108; doi:10.3390/md14060108

Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase

1
Preclinical Medicine College, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 211816, China
3
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
4
Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Long
Received: 26 February 2016 / Revised: 17 May 2016 / Accepted: 20 May 2016 / Published: 6 June 2016
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Abstract

Enzymatic hydrolysis of sodium alginate to produce alginate oligosaccharides has drawn increasing attention due to its advantages of containing a wild reaction condition, excellent gel properties and specific products easy for purification. However, the efficient commercial enzyme tools are rarely available. A new alginate lyase with high activity (24,038 U/mg) has been purified from a newly isolated marine strain, Cellulophaga sp. NJ-1. The enzyme was most active at 50 °C and pH 8.0 and maintained stability at a broad pH range (6.0–10.0) and temperature below 40 °C. It had broad substrate specificity toward sodium alginate, heteropolymeric MG blocks (polyMG), homopolymeric M blocks (polyM) and homopolymeric G blocks (polyG), and possessed higher affinity toward polyG (15.63 mM) as well as polyMG (23.90 mM) than polyM (53.61 mM) and sodium alginate (27.21 mM). The TLC and MS spectroscopy analysis of degradation products suggested that it completely hydrolyzed sodium alginate into oligosaccharides of low degrees of polymerization (DPs). The excellent properties would make it a promising tool for full use of sodium alginate to produce oligosaccharides. View Full-Text
Keywords: alginate lyase; Cellulophaga; enzymatic hydrolysis; oligosaccharides alginate lyase; Cellulophaga; enzymatic hydrolysis; oligosaccharides
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MDPI and ACS Style

Zhu, B.; Chen, M.; Yin, H.; Du, Y.; Ning, L. Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase. Mar. Drugs 2016, 14, 108.

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