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Mar. Drugs 2015, 13(8), 5334-5357; doi:10.3390/md13085334

Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum

1
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University, Otsu-200, Monobe, Nankoku, Kochi 783-8502, Japan
2
The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
3
Euglena Co., Ltd., 4th Floor, Yokohama Leading Venture Plaza, 75-1 Ono-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0046, Japan
4
School of Environmental Science and Engineering, Kochi University of Technology, Tosayamada, Kami, Kochi 782-8502, Japan
5
Department of Aquatic Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
6
Synthetic Organic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
7
Science Research Center, Kochi University, Oko-cho Kohasu, Nankoku, Kochi 783-8506, Japan
8
Center for Advanced Marine Core Research, Kochi University, Otsu-200, Monobe, Nankoku, Kochi 783-8502, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Vassilios Roussis
Received: 30 June 2015 / Revised: 30 July 2015 / Accepted: 11 August 2015 / Published: 20 August 2015
(This article belongs to the Special Issue Metabolites in Diatoms)
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Abstract

Carotenoids exert beneficial effects on human health through their excellent antioxidant activity. To increase carotenoid productivity in the marine Pennales Phaeodactylum tricornutum, we genetically engineered the phytoene synthase gene (psy) to improve expression because RNA-sequencing analysis has suggested that the expression level of psy is lower than other enzyme-encoding genes that are involved in the carotenoid biosynthetic pathway. We isolated psy from P. tricornutum, and this gene was fused with the enhanced green fluorescent protein gene to detect psy expression. After transformation using the microparticle bombardment technique, we obtained several P. tricornutum transformants and confirmed psy expression in their plastids. We investigated the amounts of PSY mRNA and carotenoids, such as fucoxanthin and β-carotene, at different growth phases. The introduction of psy increased the fucoxanthin content of a transformants by approximately 1.45-fold relative to the levels in the wild-type diatom. However, some transformants failed to show a significant increase in the carotenoid content relative to that of the wild-type diatom. We also found that the amount of PSY mRNA at log phase might contribute to the increase in carotenoids in the transformants at stationary phase. View Full-Text
Keywords: microalgae; marine diatom; transformation; PSY; carotenoid microalgae; marine diatom; transformation; PSY; carotenoid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kadono, T.; Kira, N.; Suzuki, K.; Iwata, O.; Ohama, T.; Okada, S.; Nishimura, T.; Akakabe, M.; Tsuda, M.; Adachi, M. Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 5334-5357.

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