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Mar. Drugs 2015, 13(6), 3877-3891; doi:10.3390/md13063877

Ecklonia cava Polyphenol Has a Protective Effect against Ethanol-Induced Liver Injury in a Cyclic AMP-Dependent Manner

Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan
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Author to whom correspondence should be addressed.
Academic Editor: Colin Barrow
Received: 12 March 2015 / Revised: 23 May 2015 / Accepted: 9 June 2015 / Published: 18 June 2015
(This article belongs to the Special Issue Marine Functional Food)
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Abstract

Previously, we showed that Ecklonia cava polyphenol (ECP) treatment suppressed ethanol-induced increases in hepatocyte death by scavenging intracellular reactive oxygen species (ROS) and maintaining intracellular glutathione levels. Here, we examined the effects of ECP on the activities of alcohol-metabolizing enzymes and their regulating mechanisms in ethanol-treated hepatocytes. Isolated hepatocytes were incubated with or without 100 mM ethanol. ECP was dissolved in dimethylsulfoxide. ECP was added to cultured cells that had been incubated with or without ethanol. The cells were incubated for 0–24 h. In cultured hepatocytes, the ECP treatment with ethanol inhibited cytochrome P450 2E1 (CYP2E1) expression and activity, which is related to the production of ROS when large quantities of ethanol are oxidized. On the other hand, ECP treatment with ethanol increased the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase. These changes in activities of CYP2E1 and ADH were suppressed by treatment with H89, an inhibitor of protein kinase A. ECP treatment with ethanol enhanced cyclic AMP concentrations compared with those of control cells. ECP may be a candidate for preventing ethanol-induced liver injury via regulating alcohol metabolic enzymes in a cyclic AMP-dependent manner. View Full-Text
Keywords: Ecklonia cava polyphenol; cytochrome P450 2E1; alcohol dehydrogenase; reactive oxygen species; cyclic AMP; hepatocytes Ecklonia cava polyphenol; cytochrome P450 2E1; alcohol dehydrogenase; reactive oxygen species; cyclic AMP; hepatocytes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yamashita, H.; Goto, M.; Matsui-Yuasa, I.; Kojima-Yuasa, A. Ecklonia cava Polyphenol Has a Protective Effect against Ethanol-Induced Liver Injury in a Cyclic AMP-Dependent Manner. Mar. Drugs 2015, 13, 3877-3891.

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