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Mar. Drugs 2015, 13(4), 2287-2305; doi:10.3390/md13042287

Piscidin is Highly Active against Carbapenem-Resistant Acinetobacter baumannii and NDM-1-Producing Klebsiella pneumonia in a Systemic Septicaemia Infection Mouse Model

1
Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan 811, Taiwan
2
Department of Biotechnology, Southern Taiwan University, 1 Nan-Tai Street, Yung-Kang City, Tainan County 710, Taiwan
3
School of Medicine, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei 112, Taiwan
4
Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 112, Taiwan
5
Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editor: Kellie L. Tuck
Received: 1 March 2015 / Revised: 30 March 2015 / Accepted: 1 April 2015 / Published: 14 April 2015
(This article belongs to the Special Issue Marine Peptides and Their Mimetics)
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Abstract

This study was designed to investigate the antimicrobial activity of two synthetic antimicrobial peptides from an aquatic organism, tilapia piscidin 3 (TP3) and tilapia piscidin 4 (TP4), in vitro and in a murine sepsis model, as compared with ampicillin, tigecycline, and imipenem. Mice were infected with (NDM-1)-producing K. pneumonia and multi-drug resistant Acinetobacter baumannii, and subsequently treated with TP3, TP4, or antibiotics for different periods of time (up to 168 h). Mouse survival and bacterial colony forming units (CFU) in various organs were measured after each treatment. Toxicity was determined based on observation of behavior and measurement of biochemical parameters. TP3 and TP4 exhibited strong activity against K. pneumonia and A. baumannii in vitro. Administration of TP3 (150 μg/mouse) or TP4 (50 μg/mouse) 30 min after infection with K. pneumonia or A. baumannii significantly increased survival in mice. TP4 was more effective than tigecycline at reducing CFU counts in several organs. TP3 and TP4 were shown to be non-toxic, and did not affect mouse behavior. TP3 and TP4 are able at potentiate anti-Acinetobacter baumannii or anti-Klebsiella pneumonia drug activity, reduce bacterial load, and prevent drug resistance, indicating their potential for use in combating multidrug-resistant bacteria. View Full-Text
Keywords: antimicrobial peptide; piscidin; Acinetobacter baumannii; Klebsiella pneumonia antimicrobial peptide; piscidin; Acinetobacter baumannii; Klebsiella pneumonia
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pan, C.-Y.; Chen, J.-C.; Chen, T.-L.; Wu, J.-L.; Hui, C.-F.; Chen, J.-Y. Piscidin is Highly Active against Carbapenem-Resistant Acinetobacter baumannii and NDM-1-Producing Klebsiella pneumonia in a Systemic Septicaemia Infection Mouse Model. Mar. Drugs 2015, 13, 2287-2305.

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