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Mar. Drugs 2015, 13(4), 1710-1725; doi:10.3390/md13041710

Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations

1
Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, P.O. Box 96134, Amman 11196, Jordan
2
The Jordanian Pharmaceutical Manufacturing Company (PLC), Research and Innovation Centre, P.O. Box 94, Naor 11710, Jordan
3
College of Pharmacy, Taif University, Taif 5700, Saudi Arabia
4
Department of Chemistry, Faculty of Science, Al al-Bayt University, P.O. Box 130040, Mafraq 25113, Jordan
*
Author to whom correspondence should be addressed.
Academic Editor: David Harding
Received: 10 December 2014 / Revised: 19 January 2015 / Accepted: 15 February 2015 / Published: 27 March 2015
(This article belongs to the Special Issue Advances in Marine Chitin and Chitosan)
View Full-Text   |   Download PDF [922 KB, uploaded 27 March 2015]   |  

Abstract

The objective of the present study was to prepare and characterize low molecular weight chitosan (LMWC) with different molecular weight and degrees of deacetylation (DDA) and to optimize their use in oral insulin nano delivery systems. Water in oil nanosized systems containing LMWC-insulin polyelectrolyte complexes were constructed and their ability to reduce blood glucose was assessed in vivo on diabetic rats. Upon acid depolymerization and testing by viscosity method, three molecular weights of LMWC namely, 1.3, 13 and 18 kDa were obtained. As for the DDA, three LMWCs of 55%, 80% and 100% DDA were prepared and characterized by spectroscopic methods for each molecular weight. The obtained LMWCs showed different morphological and in silico patterns. Following complexation of LMWCs with insulin, different aggregation sizes were obtained. Moreover, the in vivo tested formulations showed different activities of blood glucose reduction. The highest glucose reduction was achieved with 1.3 kDa LMWC of 55% DDA. The current study emphasizes the importance of optimizing the molecular weight along with the DDA of the incorporated LMWC in oral insulin delivery preparations in order to ensure the highest performance of such delivery systems. View Full-Text
Keywords: insulin; oral delivery; oligochitosan; low molecular weight chitosan; nanoparticles; spectroscopy; glucose; diabetic rats insulin; oral delivery; oligochitosan; low molecular weight chitosan; nanoparticles; spectroscopy; glucose; diabetic rats
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Qinna, N.A.; Karwi, Q.G.; Al-Jbour, N.; Al-Remawi, M.A.; Alhussainy, T.M.; Al-So'ud, K.A.; Omari, M.M.H.A.; Badwan, A.A. Influence of Molecular Weight and Degree of Deacetylation of Low Molecular Weight Chitosan on the Bioactivity of Oral Insulin Preparations. Mar. Drugs 2015, 13, 1710-1725.

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