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Mar. Drugs 2015, 13(3), 1466-1480; doi:10.3390/md13031466

Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa

1
Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China
2
Department of Natural Medicinal Chemistry, Jinan University, Guangzhou 510632, China
3
Department of Pharmacology, Jinan University, Guangzhou 510632, China
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Orazio Taglialatela-Scafati
Received: 18 July 2014 / Revised: 1 March 2015 / Accepted: 4 March 2015 / Published: 17 March 2015
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Abstract

A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing. View Full-Text
Keywords: Tegillarca granosa; protein; purification; characterization; antitumor activity Tegillarca granosa; protein; purification; characterization; antitumor activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lv, S.; Gao, J.; Liu, T.; Zhu, J.; Xu, J.; Song, L.; Liang, J.; Yu, R. Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa. Mar. Drugs 2015, 13, 1466-1480.

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