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Mar. Drugs 2015, 13(3), 1360-1374; doi:10.3390/md13031360

Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia

1
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2
College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, China
3
Heze Juxinyuan Food Co. Ltd., Heze 274400, China
4
Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong 226006, China
*
Author to whom correspondence should be addressed.
Academic Editor: Paola Laurienzo
Received: 15 January 2015 / Revised: 2 March 2015 / Accepted: 5 March 2015 / Published: 16 March 2015
(This article belongs to the Collection Marine Polysaccharides)
View Full-Text   |   Download PDF [1273 KB, uploaded 16 March 2015]   |  

Abstract

Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway. View Full-Text
Keywords: polysaccharide; Kjellmaniella crassifolia; anti-complement polysaccharide; Kjellmaniella crassifolia; anti-complement
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, W.; Jin, W.; Sun, D.; Zhao, L.; Wang, J.; Duan, D.; Zhang, Q. Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia. Mar. Drugs 2015, 13, 1360-1374.

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