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Mar. Drugs 2015, 13(11), 6818-6837; doi:10.3390/md13116818

Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells

1
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea
2
Department of Biomedical Engineering and Center for Marine-Integrated Biotechnology (BK21 Plus), Pukyong National University, Busan 608-737, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Takao Ojima
Received: 2 October 2015 / Revised: 3 November 2015 / Accepted: 3 November 2015 / Published: 12 November 2015
(This article belongs to the Special Issue Green Chemistry Approach to Marine Products)
View Full-Text   |   Download PDF [1690 KB, uploaded 12 November 2015]   |  

Abstract

Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine. View Full-Text
Keywords: fucoidan; fucoidanase; Streptomyces; gold nanoparticles; HeLa cells fucoidan; fucoidanase; Streptomyces; gold nanoparticles; HeLa cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Manivasagan, P.; Oh, J. Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells. Mar. Drugs 2015, 13, 6818-6837.

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