Next Article in Journal
Relative and Absolute Stereochemistry of Diacarperoxides: Antimalarial Norditerpene Endoperoxides from Marine Sponge Diacarnus megaspinorhabdosa
Next Article in Special Issue
Localization and Characterization of Ferritin in Demospongiae: A Possible Role on Spiculogenesis
Previous Article in Journal
Synthesis and Biological Evaluation of Novel 3-Alkylpyridine Marine Alkaloid Analogs with Promising Anticancer Activity
Previous Article in Special Issue
Understanding Nanocalcification: A Role Suggested for Crystal Ghosts
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2014, 12(8), 4379-4398; https://doi.org/10.3390/md12084379

Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery

Department of Food Science, College of Life Science, National Taiwan Ocean University, 2 Pei Ning Road, Keelung 20224, Taiwan
*
Author to whom correspondence should be addressed.
Received: 6 May 2014 / Revised: 16 July 2014 / Accepted: 16 July 2014 / Published: 31 July 2014
(This article belongs to the Special Issue Marine Biomaterials)
View Full-Text   |   Download PDF [2659 KB, uploaded 24 February 2015]   |  

Abstract

In this study, we developed novel chitosan/fucoidan nanoparticles (CS/F NPs) using a simple polyelectrolyte self-assembly method and evaluated their potential to be antioxidant carriers. As the CS/F weight ratio was 5/1, the CS/F NPs were spherical and exhibited diameters of approximately 230–250 nm, as demonstrated by TEM. These CS/F NPs maintained compactness and stability for 25 day in phosphate-buffered saline (pH 6.0–7.4). The CS/F NPs exhibited highly potent antioxidant effects by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing the concentration of intracellular reactive oxygen species (ROS) and superoxide anion (O2) in stimulated macrophages. The DPPH scavenging effect of CS/F NPs primarily derives from fucoidan. Furthermore, these CS/F NPs activated no host immune cells into inflammation-mediated cytotoxic conditions induced by IL-6 production and NO generation. The MTT cell viability assay revealed an absence of toxicity in A549 cells after exposure to the formulations containing 0.375 mg NPs/mL to 3 mg NPs/mL. Gentamicin (GM), an antibiotic, was used as a model drug for an in vitro releasing test. The CS/F NPs controlled the release of GM for up to 72 h, with 99% of release. The antioxidant CS/F NPs prepared in this study could thus be effective in delivering antibiotics to the lungs, particularly for airway inflammatory diseases. View Full-Text
Keywords: chitosan; fucoidan; nanoparticle; antioxidant; antibiotic; delivery chitosan; fucoidan; nanoparticle; antioxidant; antibiotic; delivery
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Huang, Y.-C.; Li, R.-Y. Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery. Mar. Drugs 2014, 12, 4379-4398.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top