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Mar. Drugs 2014, 12(6), 3218-3230; doi:10.3390/md12063218
Article

Profiling of Polar Lipids in Marine Oleaginous Diatom Fistulifera solaris JPCC DA0580: Prediction of the Potential Mechanism for Eicosapentaenoic Acid-Incorporation into Triacylglycerol

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Received: 20 February 2014; in revised form: 22 April 2014 / Accepted: 23 April 2014 / Published: 28 May 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
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Abstract: The marine oleaginous diatom Fistulifera solaris JPCC DA0580 is a candidate for biodiesel production because of its high lipid productivity. However, the substantial eicosapentaenoic acid (EPA) content in this strain would affect the biodiesel quality. On the other hand, EPA is also known as the essential health supplement for humans. EPAs are mainly incorporated into glycerolipids in the microalgal cell instead of the presence as free fatty acids. Therefore, the understanding of the EPA biosynthesis including the incorporation of the EPA into glycerolipids especially triacylglycerol (TAG) is fundamental for regulating EPA content for different purposes. In this study, in order to identify the biosynthesis pathway for the EPA-containing TAG species, a lipidomic characterization of the EPA-enriched polar lipids was performed by using direct infusion electrospray ionization (ESI)-Q-TRAP-MS and MS/MS analyses. The determination of the fatty acid positional distribution showed that the sn-2 position of all the chloroplast lipids and part of phosphatidylcholine (PC) species was occupied by C16 fatty acids. This result suggested the critical role of the chloroplast on the lipid synthesis in F. solaris. Furthermore, the exclusive presence of C18 fatty acids in PC highly indicated the biosynthesis of EPA on PC. Finally, the PC-based acyl-editing and head group exchange processes were proposed to be essential for the incorporation of EPA into TAG and chloroplast lipids.
Keywords: marine oleaginous diatom; Fistulifera solaris; lipid synthesis; eicosapentaenoic acid (EPA); PC-based acyl-editing marine oleaginous diatom; Fistulifera solaris; lipid synthesis; eicosapentaenoic acid (EPA); PC-based acyl-editing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liang, Y.; Maeda, Y.; Yoshino, T.; Matsumoto, M.; Tanaka, T. Profiling of Polar Lipids in Marine Oleaginous Diatom Fistulifera solaris JPCC DA0580: Prediction of the Potential Mechanism for Eicosapentaenoic Acid-Incorporation into Triacylglycerol. Mar. Drugs 2014, 12, 3218-3230.

AMA Style

Liang Y, Maeda Y, Yoshino T, Matsumoto M, Tanaka T. Profiling of Polar Lipids in Marine Oleaginous Diatom Fistulifera solaris JPCC DA0580: Prediction of the Potential Mechanism for Eicosapentaenoic Acid-Incorporation into Triacylglycerol. Marine Drugs. 2014; 12(6):3218-3230.

Chicago/Turabian Style

Liang, Yue; Maeda, Yoshiaki; Yoshino, Tomoko; Matsumoto, Mitsufumi; Tanaka, Tsuyoshi. 2014. "Profiling of Polar Lipids in Marine Oleaginous Diatom Fistulifera solaris JPCC DA0580: Prediction of the Potential Mechanism for Eicosapentaenoic Acid-Incorporation into Triacylglycerol." Mar. Drugs 12, no. 6: 3218-3230.


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