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A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata
Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China
College of Pharmacy, Jinan University, Guangzhou 510632, China
Guangdong Food and Drug Vocational-Technical School, Guangzhou 510663, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 March 2013; in revised form: 22 April 2013 / Accepted: 8 May 2013 / Published: 24 May 2013
Abstract: A new antitumor and antioxidant peptide (H3) was isolated from Arca subcrenata Lischke using ion exchange and hydrophobic column chromatography. The purity of H3 was over 99.3% in reversed phase-high performance liquid chromatography (RP-HPLC) and the molecular weight was determined to be 20,491.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). The isoelectric point of H3 was measured to be 6.65 by isoelectric focusing-polyacrylamide gel electrophoresis. Partial amino acid sequence of this peptide was determined as ISMEDVEESRKNGMHSIDVNH DGKHRAYWADNTYLM-KCMDLPYDVLDTGGKDRSSDKNTDLVDLFELDMVPDRK NNECMNMIMDVIDTN-TAARPYYCSLDVNHDGAGLSMEDVEEDK via MALDI-TOF/ TOF-MS and de novo sequencing. The in vitro antitumor activity of H3 was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The result indicated that H3 exhibited significant antiproliferative activity against HeLa, HepG2 and HT-29 cell lines with IC50 values of 10.8, 10.1 and 10.5 μg/mL. The scavenging percentage of H3 at 8 mg/mL to 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals were 56.8% and 47.5%, respectively.
Keywords: Arca subcrenata; peptide; antiproliferative activity; antioxidant activity
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MDPI and ACS Style
Chen, L.; Song, L.; Li, T.; Zhu, J.; Xu, J.; Zheng, Q.; Yu, R. A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata. Mar. Drugs 2013, 11, 1800-1814.
Chen L, Song L, Li T, Zhu J, Xu J, Zheng Q, Yu R. A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata. Marine Drugs. 2013; 11(6):1800-1814.
Chen, Lili; Song, Liyan; Li, Tingfei; Zhu, Jianhua; Xu, Jian; Zheng, Qin; Yu, Rongmin. 2013. "A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata." Mar. Drugs 11, no. 6: 1800-1814.